Functional Analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius Squalene-Hopene Cyclase. Neoachillapentaene, a Novel Triterpene with the 1, 5, 6-Trimethylcyclohexene Moiety Produced through Folding of the Constrained Boat Structure(Organic Che
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概要
- 論文の詳細を見る
The functions of Tyr420 and Leu607 were analyzed by constructing various site-directed mutants. The mutation at position 420 into Ala and Gly gave bicyclic α-and γ-polypodatetraene in significant amounts, but with a trace amount of tricyclic malabaricatriene. The kinetic data for and the product distribution of the Y420F mutant indicate that the major function of Tyr420 is to stabilize the 6/6-fused bicyclic cation. Mutation experiments on Leu607 demonstrate that the appropriate steric bulk size at position 607 is required to strongly bind with the product-like conformation formed during the polycyclization process. Introduction of the bulkiest Trp residue into 420 or 607 led to the production of a novel monocyclic triterpene having the (5R, 6R)-1,5,6-trimethylcyclohexene ring, named neoachillapentaene, indicating that the enzymatic cyclization proceeded via a constrained boat structure. Folding of the squalene molecule into a boat conformation by squalene cyclase has not been reported before.
- 社団法人日本農芸化学会の論文
- 2002-08-23
著者
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Sato Takenhito
Department Of Immunology Tokai University School Of Medicine
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Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
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Hoshino Tsutomu
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Hoshino Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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SASAHARA Shigehiro
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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YAMAKAMI Toshiyuki
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Sasahara S
Yamaguchi Univ. Yamaguchi Jpn
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Sato Tsutomu
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
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Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
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Yamakami Toshiyuki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sato Tsutomu
Departmenet of Physics,Faculty of Sience,Hirosaki University
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