Characterization of the Rv3378c Gene Product, a New Diterpene Synthase for Producing Tuberculosinol and (13R, S)-Isotuberculosinol (Nosyberkol), from the Mycobacterium tuberculosis H37Rv Genome
スポンサーリンク
概要
- 論文の詳細を見る
The Rv3377c and Rv3378c genes from Mycobacterium tuberculosis are specifically found in the virulent Mycobacterium species, but not in the avirulent species. The Rv3378c-encoded enzyme produced tuberculosinol 2 (5(6), 13(14)-halimadiene-15-ol), 13R-5a and 13S-isotuberculosinol 5b (5(6), 14(15)-halimadiene-13-ol) as its enzymatic products from tuberculosinyl diphosphate 3, indicating that the Rv3378c enzyme catalyzed the nucleophilic addition of a water molecule after the release of a diphosphate moiety. The three enzymatic products 2, 5a, and 5b were produced irrespective of the N- and C-terminal His-tagged Rv3378c enzymes, and of the maltose-binding protein fusion enzyme; the product distribution ratio was identical between the enzymes as 1:1 for 2:5, and 1:3 for 5a:5b. The successful separation of 5a and 5b by a chiral HPLC column provided the first complete assignments of 1H- and 13C-NMR data for 5a and 5b. The enzymatic mechanism for producing 2, 5a, and 5b is proposed here, and the optimal catalytic conditions and kinetic parameters, in addition to the divalent metal effects, are described. Site-directed mutagenesis of Asp into Asn, targeted at the DDXXD motif, resulted in significantly decreased enzymatic activity.
- 2011-01-23
著者
-
Ootsuka Takahiro
Department of Applied Biological Chemistry, Faculty of Agriculture and Graduate School of Science an
-
Takayama Kazutoshi
Department of Applied Biological Chemistry, Faculty of Agriculture and Graduate School of Science an
-
Mitsui Toshiaki
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
-
Hoshino Tsutomu
Dep. Of Applied Biological Chemistry Fac. Of Agriculture And Graduate School Of Sci. And Technol. Ni
-
Hoshino Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Nakano Chiaki
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Ootsuka Takahiro
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Hoshino Tsutomu
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
-
Mitsui Toshiaki
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Takayama Kazutoshi
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Sato Tsutomu
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
-
Sato Tsutomu
Departmenet of Physics,Faculty of Sience,Hirosaki University
関連論文
- Proteomic Studies on Protein Oxidation in Bonito (Katsuwonus pelamis) Muscle
- Screening of periodontitis with salivary enzyme tests
- Synergistic Suppressive Effect of Double Transfection of Tumor Necrosis Factor-α and Interleukin 12 Genes on Tumorigenicity of Meth-A Cells
- Characterization of the Rv3378c Gene Product, a New Diterpene Synthase for Producing Tuberculosinol and (13R, S)-Isotuberculosinol (Nosyberkol), from the Mycobacterium tuberculosis H37Rv Genome
- Jejunal Varix with Extrahepatic Portal Obstruction Treated by Embolization Using Interventional Radiology : Report of a Case
- Two cases of hepatic artery interruption after hepatopancreatobiliary surgery treated by prostaglandin E_1 infusion via the superior mesenteric artery
- Management of pancreatic mass accompanying chronic pancreatitis
- ISOLATION AND STRUCTURE OF A NEW ORGANIC ACID ACCUMULATED IN TULIP PLANT : Tulipa gesneriana
- Taka-Amylase A in the Conidia of Aspergillus oryzae RIB40
- Analysis of Secreted Proteins during Conidial Germination of Aspergillus oryzae RIB40
- New Cyclization Mechanism for Squalene : a Ring-expansion Step for the Five-membered C-ring Intermediate in Hopene Biosynthesis
- Photon-, Reductant-, and Oxidant-Induced Substitution of a CO Ligand on closo-[Cp^*_2Fe_3S_2(CO)_3](Cp^*=η^5-C_5Me_5)
- Role of positron emission tomography with 2-deoxy-2-[^F]fluoro-D-glucose in evaluating the effects of arterial infusion chemotherapy and radiotherapy on pancreatic cancer
- Positron emission tomography with 2-deoxy-2-[^F] fluoro-D-glucose for diagnosis of intraductal papillary mucinous tumor of the pancreas with parenchymal invasion
- Acute Emphysematous Cholecystitis Preceded by Symptoms of Ileus : Report of a Case
- Pseudodeoxyviolacein, a New Red Pigment Produced by the Tryptophan Metabolism of Chromobacterium violaceum
- A New Metabolite of Tryptophan, Chromopyrrolic Acid, Produced by Chromobacterium violaceum
- Purification of β-Glucan Synthase II from Suspension-cultured Rice Cells
- Temperature Dependence of the Raman Shift of the A_ mode for NdGaO_3 Crystal
- The First-Order Raman Spectra and Lattice Dynamics for YAlO_3 Crystal
- The First-Order Raman Spectra and Lattice Dynamics for YAlO_3 Crystal
- Long-term Outcome of Systemic Cyclosporine Treatment Following Penetrating Keratoplasty
- Long-Term Effects of Topical Cyclosporine A Treatment after Penetrating Keratoplasty
- Effect of Green Tea on Volatile Sulfur Compounds in Mouth Air
- Structure and Function of the Golgi Complex in Rice Cells : Characterization of Golgi Membrane Glycoproteins
- Localization of Binding Sites for Peanut, Ulex europaeus, and Castor Bean Lectin in the Rice Golgi Complex
- Preparation of Golgi Membrane Fractions Containing Lectin-binding Sites from Suspension-cultured Rice Cells
- Isolation and Characterization of Golgi Membranes from Suspension-cultured Cells of Rice (Oryza sativa L.)
- Biosynthesis of Violacein: a Novel Rearrangement in Tryptophan Metabolism with a 1,2-Shift of the Indole Ring(Organic Chemistry)
- Comparison of the Oral Flora of Independent Elderly and Those Requiring Nursing Assistance
- Effects of Secondary Organ Failure on Compensation of Acute Heart Failure in Patients with Myocardial Infarct and Dilated Cardiomyopathy : COMPENSATORY MECHANISMS AND THEIR LIMITATIONS IN HEART FAILURE
- Purification of Phosphoinositol Kinase from Suspension-cultured Cells of Rice (Oryza sativa L.)
- Conductance Peak at Zero-Bias in Ag-SiO-Bi_2Sr_2CaCu_2O_ Planar Tunnel Junctions
- Hirschsprung's Disease in an Adult Patient with Familial Occurrence : Report of a Case
- P-235 ENZYMATIC SYNTHESES OF SESQUI-, DI-AND SESTER-TERPENE SKELTONS BY SQUALENE CYCLASE
- Characterization of Isofenphos Hydrolases from Arthrobacter sp. : Strain B-5
- Biodegradation of Organophosphorus Insecticides by Bacteria Isolated from Turf Green Soil
- Temperature Dependence of the Linewidth of the First-Order Raman Spectra for CaWO_4 Crystal
- Emulsifying Properties of Protein-Polysaccharide Complexes and Hybrids(Food & Nutrition)
- Formations of (5-Hydroxy)indole S-(-)-lactic Acid, N-Acetyl-5-hydroxy-L-tryptophan, and (5-Hydroxy)indole Carboxylic Acid in the Metabolism of Tryptophan and 5-Hydroxy-tryptophan by Chromobacterium violaceum
- Laparoscopic right hemihepatectomy for a case of polycystic liver disease with right predominance
- Resection of a Solitary Pancreatic Metastasis from Renal Cell Carcinoma with a Gallbladder Carcinoma : Report of a Case
- Synthesis, Biological Activity, and Metabolism of 8', 8', 8'-Trideuteroabscisic Acid
- Temperature Dependence of the Ag+Bg-Mode of Raman Shift for CaWO_4 Crystal
- Biosynthesis of Violacein : Intact Incorporation of the Tryptophan Molecule on the Oxindole Side, with Intramolecular Rearrangement of the Indole Ring on the 5-Hydroxyindole Side
- Rhus javanica var. chinensis as a New Plant Origin of Propolis from Okayama, Japan
- Biosynthesis of Violacein : Evidence for the Intermediacy of 5-Hydroxy-L-tryptophan and the Structure of a New Pigment, Oxyviolacein, Produced by the Metabolism of 5-Hydroxytryptophan(Organic Chemistry)
- Painful hepatic hemangioma : report of a case with an emphasis on sonographic findings
- Characterization of the Rv3378c Gene Product, a New Diterpene Synthase for Producing Tuberculosinol and (13R, S)-Isotuberculosinol (Nosyberkol), from the Mycobacterium tuberculosis H37Rv Genome
- A resected case of a small hepatocellular carcinoma developing within the bile duct
- Cholangiocarcinoma coincident with schistosomiasis japonica
- Appraisal of Intra-Arterial Infusion of Prostaglandin E_1 in Patients Undergoing Major Hepatic Resection -Report of Four Cases
- Enhanced cytokine production in peripheral blood moonocytes following hepatic resetion
- Catalytic Function of the Residues of Phenylalanine and Tyrosine Conserved in Squalene-Hopene Cyclases(Organic Chemistry)
- Sterol Biosynthesis by a Prokaryote : First in Vitro Identification of the Genes Encoding Squalene Epoxidase and Lanosterol Synthase from Methylococcus capsulatus
- P-295 NOVEL DITERPENE SYNTHASES FROM MYCOBACTERIUM TUBERCULOSIS H37RV
- Purification and Characterization of Phosphatidylinositol-specific Phospholipase C in Suspension-cultured Cells of Rice (Oryza sativa L.)
- Sugar Nucleotide Contents in Suspension-cultured Rice Cells(Biological Chemistry)
- Novel Compounds of Octahydroheptaprenyl Mycolic Acyl Ester and Monocyclic C_-Terpene, Heptaprenylcycline B, from Non-Pathogenic Mycobacterium Species
- Temperature Dependence of the Linewidth of the First-Order Raman Spectrum for TeO_2 Crystal
- Inhibitory Activity of 8-Azadecalin Derivatives towards 2,3-Oxidosqualene : Lanosterol Cyclases from Baker's Yeast and Pig's Liver
- Functional Analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius Squalene-Hopene Cyclase. Neoachillapentaene, a Novel Triterpene with the 1, 5, 6-Trimethylcyclohexene Moiety Produced through Folding of the Constrained Boat Structure(Organic Che
- Polarization-Dependent Phase-Conjugate Reflectivity in Randomly Oriented Saturable Absorbers : Effects of Direction of Transition Dipole Moment Associated with Excited-State Absorption
- EXPANSION CHARACTERISTICS OF MONTMORILLONITE AND SAPONITE UNDER VARIOUS RELATIVE HUMIDITY CONDITIONS
- Functional Analysis of the DXDDTA Motif in Squalene-Hopene Cyclase by Site-directed Mutagenesis Experiments : Initiation Site of the Polycyclization Reaction and Stabilization Site of the Carbocation Intermediate of the Initially Cyclized A-Ring
- Evaluation of Chronic Toxicity of Water Lead for Carp Cyprinus carpio Using Its Blood 5-Aminolevulinic Acid Dehydratase
- Temperature Dependence of the Linewidth of the First-Order Raman Spectra for Aragonite Crystal
- The First-Order Raman Spectra and Lattice Dynamics for CdWO_4 Crystal
- Lattice Dynamics and Temperature Dependence of the Linewidth of the First-Order Raman Spectra for Sintered Hexagonal GeO_2 Crystalline
- Contribution of Absorptive and Refractive Gratings to Mixed Holograms Using Xanthene-Dye-Doped Films
- Polarization-Dependent Light-Grated Phase Conjugation in Methyl-Orange Doped Polymer Film
- Method not Requiring Mercuric Chloride for the Determination of Activity of 5-Aminolevulinic Acid Dehydratase in Blood of Carp Cyprinus carpio
- Cytotoxicity and Mutagenicity to Human Adult Gingival Cells of Sodium Fluoride Releasing Devices
- Relationship between bone mineral loss and urinary fluoride concentration in postmenopausal Japanese women
- Evaluation of Erythrocyte 5-Aminolevulinic Acid Dehydratase Activity in the Blood of Carp Cyprinus carpio as an Indicator in Fish with Water Lead Pollution
- Site-directed Mutagenesis Experiments on the Putative Deprotonation Site of Squalene-hopene Cyclase from Alicyclobacillus acidocaldarius
- Conversion from Tryptophan Precursor into Violacein Pigments by a Cell-free System from Chromobacterium violaceum
- Significance of Biological Samples as Biomarkers of Fluoride Exposure
- Research Regarding the Health Care of Dentists : Stress Evaluation Using Heart Rate Fluctuation Spectral Analysis and Lifestyle Habits
- Temperature Dependence of the Linewidth of the First-Order Raman Spectrum for SnO_2 Crystal
- Lattice Dynamics and Temperature Dependence of the First-Order Raman Spectra for PbMoO_4 Crystal : Condensed Matter: Structure, etc.
- Temperature Dependence of Raman Band of SnO_2 Crystal
- Elution Behavior Analysis of Starch Degrading Enzymes During Rice Cooking with Specific Antibodies
- Identification of the SGR6065 gene product as a sesquiterpene cyclase involved in (+)-epicubenol biosynthesis in Streptomyces griseus
- Elution Behavior Analysis of Starch Degrading Enzymes During Rice Cooking with Specific Antibodies
- Biosynthesis of Violacein: Origins of Hydrogen, Nitrogen and Oxygen Atoms in the 2-Pyrrolidone Nucleus
- DISTENSIBILITY OF CEREBRAL VESSELS IN RESPONSE TO ACUTE HYPERTENSION IN CATS
- Surface Mannan from Glomerella cingulata
- Characteristics of Opaque and Translucent Parts of High Temperature Stressed Grains of Rice
- Successful Treatment with Entecavir for Reactivation of Hepatitis B Virus Following Systemic Chemotherapy in a Hepatitis B Surface Antigen-negative Patient with Colorectal Cancer