Analysis of Secreted Proteins during Conidial Germination of Aspergillus oryzae RIB40
スポンサーリンク
概要
- 論文の詳細を見る
To broaden our understanding of extracellular proteins of Aspergillus oryzae at the conidial germination stage, analyses of the secreted proteins during germination were carried out. Taka-amylase A (TAA), glucoamylase (GLAA), and aspergillopepsin A (PEPA) were identified as the main products by peptide mass fingerprinting. TAA and PEPA were detected simultaneously with the formation of germ tubes. With the development of germination, the pH of the medium fell from 5.5 to 3.5. The secreted PEPA had a pro-sequence and likely shifted from 42 kDa to 41 kDa below pH 4.6, indicating that the precursor of PEPA was secreted and underwent pH-dependent processing. Furthermore, the 41 kDa protein was trapped by the addition of pepstatin A, the specific inhibitor of PEPA, suggesting that the maturation of pro-PEPA was a stepwise autoprocessing upon acidification of the medium and itself was an intermediate of the processing. It was implied that PEPA plays an important role at the early germination stage.
- 社団法人 日本農芸化学会の論文
- 2004-12-23
著者
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Sato Tsutomu
Department of Oral Health, Nippon Dental University School of Life Dentistry at Tokyo
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Nguyen Cong
Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
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ZHU Li
Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences a
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TAKEUCHI Michio
Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
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Zhu Li
Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
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Takeuchi Michio
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
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Takeuchi Michio
Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
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Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
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Sato Tsutomu
Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
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Sato Tsutomu
Department Of Agricultural Chemistry Faculty Of Agriculture Yamaguchi University
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Sato Tsutomu
Departmenet of Physics,Faculty of Sience,Hirosaki University
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