Molecular Cloning of ocpO Encoding Carboxypeptidase O of Aspergillus oryzae IAM2640

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概要

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• Carboxypeptidase O from Aspergillus oryzae IAM2640 is a serine-type carboxypeptidase. In this study, we cloned and sequenced cDNA and genomic DNA carrying ocpO encoding carboxypeptidase O. The results showed that the length of ocpO was 1,816 bp, and the open reading frame encoded a putative preproenzyme composed of 472 amino acid residues of the mature carboxypeptidase O and an additional N-terminal sequence of 50 amino acid residues. A BLASTN search revealed that a gene, AO090020000351, in A. oryzae RIB40, which is strain used in genome-wide sequencing, is a homolog of ocpO. The difference between AO090020000351 and ocpO was only one nucleotide. The difference caused substitution of Ala for Pro at the 277th position of the enzyme; therefore the protein encoded by AO090020000351 was overproduced and purified. The purified protein showed enzymatic properties similar to carboxypeptidase O, indicating that carboxypeptidase O and protease encoded by AO090020000351 are same enzyme.

• 2010-05-23

著者

• Ishida Hiroki

  Gekkeikan Sake Co. Ltd.

• MORITA HIROTO

  Department of Urology, Nihon University School of Medicine

• MIZUNO Takeshi

  School of Agriculture, Nagoya University

• YAMAGATA Youhei

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• TAKEUCHI Michio

  Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology

• KUSUMOTO Ken-Ichi

  National Food Research Institute, National Agriculture and Food Research Organization (NARO)

• Kusumoto K

  National Food Research Institute

• KURIYAMA Ken-Ichi

  Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Techno

• AKIYAMA Noritaka

  Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Techno

• OKAMOTO Ayako

  Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Techno

• KOIDE Yoshinao

  Amano Enzyme Inc.

• Morita Hiroto

  Department Of Urology Nihon University School Of Medicine

• Yamagata Y

  Tohoku Univ. Sendai Jpn

• Kuriyama Ken-ichi

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Takeuchi Michio

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Takeuchi Michio

  Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology

• Yamagata Youhei

  Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.

• Yamagata Youhei

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Yamagata Youhei

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• KUSUMOTO Kenichi

  National Food Research Institute

• Kusumoto Ken-ichi

  National Food Research Institute

• Akiyama Noritaka

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Morita Hiroto

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Okamoto Ayako

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

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