Alternative Processing of Proproteins in Aspergilli kexB Gene Disruptants under Hyperosmotic Conditions
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概要
- 論文の詳細を見る
Disruption of the kexB gene encoding a subtilisin-like processing protease in Aspergillus oryzae and Aspergillus nidulans led to remarkable morphological defects, and these phenotypes were suppressed under hyperosmotic conditions. In this study, we investigated to determine whether non-KexB proteases might complement the in vivo function of KexB in the two Aspergillus kexB disruptants. Neither overexpression of opsA or opsB encoding A. oryzae aspartyl proteases homologous to yeast yapsins (YPS1/2) suppressed the kexB mutation, although yapsins are multicopy suppressors for the yeast kex2 mutation. A. nidulans and A. oryzae kexB disruptants grown under hyperosmotic conditions processed a recombinant fusion protein carrying a synthetic dibasic processing site (Lys-Arg) although the disruptants grown under normal growth conditions did not cleave the site. These results suggest that the two Aspergilli have other potential processing proteases that are induced and/or activated under hyperosmotic conditions and consequently complement, at least in part, the in vivo function of KexB.
- 社団法人 日本農芸化学会の論文
- 2009-01-23
著者
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GOMI Katsuya
Laboratory of Bioindustrial Genomics, Graduate School of Agricultural Science, Tohoku University
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FURUKAWA Kentaro
Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University
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ABE Keietsu
Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University
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FUJIOKA Tomonori
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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MIZUTANI Osamu
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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ICHIYANAGI Shunsuke
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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MATSUDA Yoshihiko
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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TOKUOKA Masafumi
Laboratory of Bioindustrial Genomics, Department of Bioindustrial Informatics and Genomics, Graduate
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YAMAGATA Youhei
Laboratory of Enzymology, Department of Molecular and Cell Biology, Graduate School of Agricultural
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Abe Keietsu
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Gomi Katsuya
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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MIZUTANI Osamu
National Research Institute of Brewing
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Yamagata Youhei
Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.
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Tokuoka Masafumi
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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Furukawa Kentaro
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Fujioka Tomonori
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Matsuda Yoshihiko
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Ichiyanagi Shunsuke
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Yamagata Youhei
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Mizutani Osamu
National Res. Inst. Of Brewing
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Mizutani Osamu
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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Gomi Katsuya
Laboratory Of Bioindustrial Cenomics Graduate School Of Agricultural Science Tohoku University
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