Novel Reporter Gene Expression Systems for Monitoring Activation of the Aspergillus nidulans HOG Pathway

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概要

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• The Aspergillus nidulans high-osmolarity glycerol response (AnHOG) pathway is involved in osmoadaptation. We found that fludioxonil, a fungicide, causes improper activation of HogA mitogen-activated protein kinase (MAPK) in A. nidulans. Here we present novel reporter systems for monitoring activation of the AnHOG pathway. The promoter region of gfdB (glycerol-3-phosphate dehydrogenase), whose expression depends on the presence of HogA, was fused to a β-glucuronidase uidA gene (GUS) to construct the reporter, which was introduced into A. nidulans wild type and hogAΔ. Increased GUS activity was detected in the wild type only when it was treated with high osmolarity or fludioxonil, while reporter activity was scarcely stimulated in the hogAΔ mutant. These results indicate that the reporter activity is controlled via HogA activation. Furthermore, we present possible applications of the reporter systems in screening new antifungal compounds.

• 社団法人 日本農芸化学会の論文

著者

• Kobayashi Tetsuo

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• MIZUNO Takeshi

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• Hagiwara Daisuke

  Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University

• FURUKAWA Kentaro

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• ABE Keietsu

  Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University

• Yoshimi Akira

  New Industry Creation Hatchery Center, Tohoku University

• Furukawa Takako

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• HOSHI Yukiko

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• SATO Natsuko

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• FUJIOKA Tomonori

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

• MIZUTANI Osamu

  Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University

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