Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli

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概要

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• Analysis of the nucleotide sequence of the meso-2,3-butanediol dehydrogenase (D-acetoin forming)(BDH) gene of Klebsiella pneumoniae IAM1063 revealed that it contains an open reading frame of 768 bp encoding a polypeptide of 256 amino acid residues with a molecular weight of 26,591 Da. The amino acid sequence deduced from the nucleotide sequence of the BDH gene is consistent with the amino-terminal and carboxyl-terminal amino acid sequences, amino acid composition, and molocular weight of purified BDH. The amino acid sequences of BDHs from K. pneumoniae IAM1063 and Klebsiella terrigena VTT-E-74023 showed 92.9% homology in the firs 196 amino acid residues, but no homology elsewhere. Moreover, the BDH of K. pneumoniae IAM1063 was 15 amino acid residues longer than that of K. terrigena VTT-E-74023 showed 92.9% homology in the first 196 amino acid residues, but no homology elsewhere. Moreover, the BDH of K. pneumonia IAM10630 was 15 amino acid residues longer than that of K. terrigena VTT-E-74023. The caracteristics of the BDH from Escherichia coli JM109/pBUD119 (containing the meso-BDH gene) coincided with that of the BDH from the gene source strain (K. pneumoniae IAM1063). Furthermore, the BDHs from K. pneumoniae IAM1063 and K. terrigena VTT-E-74023 showed similar catalytic properties. This suggests that the downstream of Gln at position 196 in the amino acid sequence is unrelated to BDH activity.

• 社団法人日本生物工学会の論文
• 1997-01-25

著者

• Kobayashi Tetsuo

  Laboratory of Gene Regulation, Graduate School of Bioagricultural Sciences, Nagoya University

• Ui Sadaharu

  Division of Biotechnology Yamanashi Univ.

• Ui Sadaharu

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Ui Sadaharu

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• Kudo Toshiaki

  Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)

• Ui Sadaharu

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Ui Sadaharu

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• MIMURA Akio

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• OKAJIMA YOSHIFUMI

  Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University

• KANAI HARUHIKO

  Laboratory of Microbiology, the Institute of Physical and Chemical Research(RIKEN)

• Takio K

  Enviromental Molecular Biology Lab. Riken

• Kudo T

  Inst. Physical And Chemical Res.(riken) Saitama Jpn

• Kudo T

  Riken Saitama Jpn

• Kudo Toshiaki

  International Graduate School Of Arts And Sci. Yokohama City Univ.

• Kudo Toshiaki

  Yokohama City Univ. Kanagawa Jpn

• Mimura A

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Mimura A

  Hitachi Ltd. Ibaraki Jpn

• Mimura A

  Univ. Of Yamanashi Yamanashi Jpn

• Mimura Akiko

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi

• Kobayashi Tetsuo

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Okajima Yoshifumi

  Department Of Appleid Chemistry And Biotechnology Faculty Of Engineering Yamanashi University

• Kudo Toshiaki

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research

• Kanai Haruhiko

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Kobayashi T

  Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)

• Kobayashi T

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Kobayashi Tetsuo

  Laboratory Of Gene Regulation Graduate School Of Bioagricultural Sciences Nagoya University

• Mimura Akio

  Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University

• Ui S

  Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O

• Kobayashi Tetsuo

  Laboratory of Agricultural Meteorology, Faculty of Agriculture, Kyushu University

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