Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
スポンサーリンク
概要
- 論文の詳細を見る
Klebsiella pneumoniae IAM 1063 forms 2,3-butanediol consisting of a mixture of the mseo- and L-isomers from glucose. A gene fragment including genes coding for three enzymes (ALS, ALDC, and meso-BDH (D-AC forming)) involved in the formation of meso-BD of K. pneumoniae IAM 1063 was cloned in Escherichia coli JM109 after its insertion into pUC118. The resulting E. coli JM109/pBDO118 was cultured in LB medium containing glucose and formed only a meso-isomer with no contamination of the L-form. Various culture conditions for the production of meso-BD were tested. The highest meso-BD yield of 27% (w/w, 10.7 g/l) against intially added glucose was obtained in a LB culture shaken for 24 h at 30℃ in the presence of 4% glucose. However, re-conversion of meso-BD to AC occurred with time. The highest productivity of meso-BD was 17.7 g/l, which was obtained using an initial glucose concentration of 10%.
- 社団法人日本生物工学会の論文
- 1997-09-25
著者
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Ui Sadaharu
Division of Biotechnology Yamanashi Univ.
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Ui Sadaharu
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Ui Sadaharu
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
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Kudo Toshiaki
Environmental Molecular Biology Lab. :graduate School Of Integrated Science Yokohama City University
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Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)
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Ui Sadaharu
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
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MIMURA Akio
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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OKAJIMA YOSHIFUMI
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University
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KANAI HARUHIKO
Laboratory of Microbiology, the Institute of Physical and Chemical Research(RIKEN)
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Takio K
Enviromental Molecular Biology Lab. Riken
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Kudo T
Riken Saitama Jpn
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Kudo Toshiaki
International Graduate School Of Arts And Sci. Yokohama City Univ.
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Kudo Toshiaki
Yokohama City Univ. Kanagawa Jpn
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Mimura A
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
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Mimura A
Hitachi Ltd. Ibaraki Jpn
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Mimura A
Univ. Of Yamanashi Yamanashi Jpn
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Mimura Akiko
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Yamanashi
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Okajima Yoshifumi
Department Of Appleid Chemistry And Biotechnology Faculty Of Engineering Yamanashi University
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Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research
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Kanai Haruhiko
Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)
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Mimura Akio
Department Of Applied Chemistry & Biotechnology Faculty Of Engineering Yamanashi University
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Ui S
Division Of Biotechnology Interdisciplinary Graduate School Of Medicine And Engineering University O
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