Cultivation of Thermophilic Methanogen KN-15 on H_2-CO_2 under Pressurized Conditions

概要

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Cultivation of the thermophilic methanogen KN-15 was carried out under pressurized batch and continuous conditions. In pressurized batch culture, both the turning point at which cell growth changed from exponential to linear and the growth rate during the linear growth phase increased with the rise of total pressure of the gas phase in the fermentor. The cell concentration reached 18.5 g dry cell/l after 10 h of batch cultivation under 3.0×10^5 Pa pressurized conditions. Under pressurized continuous conditions, it was also observed that the cell concentration and cell productivities increased with the rise of total pressure of the gas phase. Cell and methane productivities of 3.0 g dry cell/l/h and 1.28 mol/l/h, respectively, were achieved in 3.0×10^5 Pa pressurized continuous culture. According to the results from the Monod model application, the achievable cell productivities (V_<max>) and the Monod type saturation constant for cell productivities (K'_s) were 12.8 g dry cell/l/h and 9.7×10^5 Pa, respectively.
社団法人日本生物工学会の論文
1992-06-25