Enhancement of Protein Synthesis in Continuous-Flow, Cell-Free System by Improvement of Membrane Permeation

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概要

• 論文の詳細を見る

• The productiveity of the continuous-flow, cell-free protein synthesis system (CFCF-system) is inferior compared to that in the cell-free batch system.By comparing the synthesized protein present between in the reaction chamber and in the fed-out solution in the CFCF-system, it appeared that much of the sythesized protein remained in the reaction chamber. Addition of a nonionic detergent, n-dodecyl β-D-maltoside, to the feeding solution resulted in enhancement of the productivity in the CFCF-system which indicated that the permeability of the membrane to the protein might be one of the most influential factors affecting the productivity of the CFCF-system.

• 公益社団法人日本生物工学会の論文
• 1995-10-25

著者

• Niwano Mitsuru

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Kitaoka Y

  Kobe Steel Ltd. Kobe Jpn

• Nishimura Norihiro

  Biotechnology Research Laboratory Kobe Steel Ltd.

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.,

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.

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