Cell-Free System Derived from Heat-Shocked Escherichia coli : Synthesis of Enzyme Protein Possessing Higher Specific Activity

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概要

• 論文の詳細を見る

• Heat-shocked S30 extract (HS-S30 extract) was prepared from cells of Escherichia coli strain Q13 exposed to elevated temperatures (from 37℃ to 42℃) for 30 min. In a cell-free system with HS-S30 extract, the synthesized CAT protein had higher specific acitivity than that synthesized by a cell-free system with S30 extract prepared from Q13 cells incubated at 37℃. SDS-PAGE analysis showed that the heat-shock proteins, GroEL and DnaK, which are known to be molecular chaperones, were significantly increased in the HS-S30 extract. The addition of GroEL or DnaK to the S30 extract system increased the specific activity of the synthesized CAT protein. Heat-shock induction thus offers and effective method of modifying E. coli cell extracts.

• 公益社団法人日本生物工学会の論文
• 1995-02-25

著者

• Niwano Mitsuru

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Kitaoka Y

  Kobe Steel Ltd. Kobe Jpn

• Nishimura Norihiro

  Biotechnology Research Laboratory Kobe Steel Ltd.

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.,

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.

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• Cell-Free System Derived from Heat-Shocked Escherichia coli : Synthesis of Enzyme Protein Possessing Higher Specific Activity
• Utilization of Copper Ion as a Ribonuclease Inhibitor in a Cell-Free Protein Synthesis System

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