Utilization of Copper Ion as a Ribonuclease Inhibitor in a Cell-Free Protein Synthesis System

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概要

• 論文の詳細を見る

• The degradation of mRNA in a cell-free coupled transcription and translation system derived from Escherichia coli was prevented by the addition of 0.5mM copper ion. Neither the transcription nor the translation was influenced by the presence of copper ion at this concentration, whereas the transcription was inhibited by a copper ion concentration exceeding 1.0mM, and the translation by a concentration of 1.0mM or more. In the cell- free system conducted with mRNA as a template, the amount of protein synthesized increased markedly in the presence of 0.5 mM copper ion. These results indicate that copper ion can stabilize cell-free protein synthesis systems by preventing mRNA degradation.

• 公益社団法人日本生物工学会の論文
• 1994-08-25

著者

• Niwano Mitsuru

  Biotechnology Research Laboratory Kobe Steel Ltd.

• Kitaoka Y

  Kobe Steel Ltd. Kobe Jpn

• Nishimura Norihiro

  Biotechnology Research Laboratory Kobe Steel Ltd.

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.,

• KITAOKA YOSHIHISA

  Biotechnology Research Laboratory, Kobe Steel Ltd.

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• Enhancement of Protein Synthesis in Continuous-Flow, Cell-Free System by Improvement of Membrane Permeation
• Cell-Free System Derived from Heat-Shocked Escherichia coli : Synthesis of Enzyme Protein Possessing Higher Specific Activity
• Utilization of Copper Ion as a Ribonuclease Inhibitor in a Cell-Free Protein Synthesis System

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