Purification and Characterization of α-Amylase from Hyperthermophilic Archaeon Thermococcus profundus, Which Hydrolyzes Both α-1, 4 and α-1, 6 Glucosidic Linkages
スポンサーリンク
概要
- 論文の詳細を見る
A hyperthermophilic archaeon Thermococcus profundus produced two α-amylases, S and L. α-Amylase L was purified to homogeneity and characterized in the present study. The enzyme hydrolyzed not only α-1, 4 linkages of amylose and starch, but also α-1, 6 linkages of pullulan. It exhibited maximal activity towards both starch and pullulan at pH 5.5. The temperatures for optimal amylolytic and pullulolytic activities were 80℃ and 90℃, respectively, and the half-lives for these activities at 90℃ in the presence of 5 mM Ca^<2+> were 4h and 6.7h, respectively. The enzyme activity was totally inhibited by 1 mM N-bromosuccinimide. The molecular mass of the enzyme was estimated to be 43, 000 Da by SDS-polyacrylamide gel electrophoresis. α-Amylase L hydrolyzed soluble starch, amylose, amylopectin and glycogen to yield glucose and maltose as the predominant products. The amylase also hydrolyzed pullulan and panose to yield the same products.
- 社団法人日本生物工学会の論文
- 1998-10-25
著者
-
Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research(riken)
-
AKIBA TERUHIKO
Laboratory of Microbiology, The Institute of Physical and Chemical Research
-
Kwak Yi
Korea Ginseng & Tobacco Research Institute Youseong
-
Kudo Toshiaki
Laboratory Of Microbiology The Institute Of Physical And Chemical Research
-
Akiba Teruhiko
Laboratory Of Microbiology The Institute Of Physical And Chemical Research
関連論文
- PA-42 An alternative pathway of primary metabolism of termite gut protists inferred from meta-EST analysis(Symbiosis/Cell to cell interaction,Poster presentation A)
- Effect of Temporal Sample Preservation on the Molecular Study of a Complex Microbial Community in the Gut of the Termite Microcerotermes sp
- Identification and in situ Detection of Two Lineages of Bacteroidales Ectosymbionts Associated with a Termite Gut Protist, Oxymonas sp.
- Fungal Community Analysis of Fungus Gardens in Termite Nests
- Isolation and Detection of Methanogens from the Gut of Higher Termites
- Gut of Higher Termites as a Niche for Alkaliphiles as Shown by Culture-Based and Culture-Independent Studies
- Nitrogen Fixation Genes Expressed in the Symbiotic Microbial Community in the Gut of the Termite Coptotermes formosanus
- Molecular Phylogeny of Symbiotic Basidiomycetes of Fungus-growing Termites in Thailand and Their Relationship with the Host(Microbiology & Fermentation Technology)
- Diverse Bacteria Related to the Bacteroides Subgroup of the CFB Phylum within the Gut Symbiotic Communities of Various Termites(Microbiology & Fermentation Technology)
- Purification and Characterization of L-2,3-Butanediol Dehydrogenase of Brevibacterium saccharolyticum C-1012 Expressed in Escherichia coli(Microbiology & Fermentation Technology)
- Characterization of the NADH-Linked Acetylacetoin Reductase/2, 3-Butanediol Dehydrogenase Gene from Bacillus cereus YUF-4
- Cloning, Expression and Nucleotide Sequence of the L-2, 3-Butanediol Dehydrogenase Gene from Brevibacterium saccharolyticum C-1012
- Molecular Generation of an Escheichia coli Srain Pproducing Only the meso-Isomer of 2,3-Butanediol
- Sequence Analysis of the Gene for and Characterization of D-Acetoin Forming meso-2,3-Butanediol Dehydrogenase of Klebsiella pneumoniae Expressed in Escherichia coli
- Cloning, Nucleotide Sequence, and Hyperexpression of α-Amylase Gene from an Archaeon, Thermococcus profundus
- Preparation of a Chiral Acetoinic Compound Using Transgenic Escherichia coli Expressing the 2,3-Butanediol Dehydrogenase Gene
- An Alkaliphilic and Xylanolytic Paenibacillus Species Isolated from the Gut of a Soil-Feeding Termite
- A shaE Deletion Mutant Showed Lower Na^+ Sensitivity Compared to Other Deletion Mutants in the Bacillus subtilis Sodium/Hydrogen Antiporter (Sha) System
- Molecular ecology of termite-microorganisms symbiotic systems(Symposium on Various Interactions between Animal and Microorganism)
- Nucleotide Sequence of the Xylanase A Gene of Alkalophilic Bacillus sp. Strain C-125(Microbiology & Fermentation Industry)
- Extracellular Production of Alkaline Xylanase of Alkalophilic Bacillus sp. by Escherichia coli Carrying pCX311
- Molecular Cloning and Expression of β-Isopropylmalate Dehydrogenase Gene from Alkalophilic Bacillus in Escherichia coli
- Molecular Cloning, Nucleotide Sequence and Expression in Escherichia coli of Hyperthermophilic Glutamate Dehydrogenase Gene from Thermococcus profundus
- Properties of Glutamate Dehydrogenase and Its Involvement in Alanine Production in a Hyperthermophilic Archaeon, Thermococcus Profundus^1
- Location of F_1 ATPase-like Genes on the Physical Map of the Bacillus subtilis 168 Chromosome
- Crystal Structure of meso-2,3-Butanediol Dehydrogenase in a Complex with NAD^+ and Inhibitor Mercaptoethanol at 1.7 A Resolution for Understanding of Chiral Substrate Recognition Mechanisms
- Carbon mineralization by termites in tropical forests, with emphasis on fungus combs
- Isolation and Characterization of Dibenzofuran-degrading Actinomycetes : Analysis of Multiple Extradiol Dioxygenase Genes in Dibenzofuran-degrading Rhodococcus Species(Biochemistry & Molecular Biology)
- Physiological Properties of a Neutralo-sensitive Mutant Derived from Facultative Alkaliphilic Bacillus sp. C-125
- Characterization of a Mutation Responsible for an Alkali-sensitive Mutant, 18224, of Alkaliphilic Bacillus sp. strain C-125
- Characteristics of Alkali-sensitive Mutants of Alkaliphilic Bacillus sp. Strain C-125 That Show Cellular Morphological Abnormalities
- Phylogenetic and Morphological Diversity of Bacteroidales Members Associated with the Gut Wall of Termites
- Two Sets of Biphenyl and PCB Degradation Genes on a Linear Plasmid in Rhodococcus erythropolis TA421
- A Gram-positive Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus eryth-ropolis Strain TA421, Isolated from a Termite Ecosystem
- Purification and Characterization of α-Amylase from Hyperthermophilic Archaeon Thermococcus profundus, Which Hydrolyzes Both α-1, 4 and α-1, 6 Glucosidic Linkages
- Extracellular Production of Human Immunoglobulin ε-Chain/γ1-Chain Chimeric Fc Polypeptide by Escherichia coli
- Extracellular Production of Human Tumor Necrosis Factor-α by Escherichia coli Using a Chemically-synthesized Gene(Biological Chemistry)
- Cloning and Characterization of the Azurin iso-1 Gene, Concerned with the Electron Transport Chain Involved in Methylamine/Methanol Oxidation in the Obligate Methylotroph Methylomonas sp. Strain J
- Genetic Organization and Characterization of the mau Gene Cluster, Which Concerned the Initial Step of Electron Transport Chains Involved in Methylamine Oxidation of the Obligate Methylotroph Methylomonas sp. Strain J
- Physiological Properties of Two Pseudomonas mendocina Strains Which Assimilate Styrene in a Two-Phase (Solvent-Aqueous) System under Static Culture Conditions
- Production of 3-Vinylcatechol and Physiological Properties of Pseudomonas LF-3, Which Can Assimilate Styrene in a Two-phase (Solvent-Aqueous) System
- Functional Expression of Three Rieske Non-Heme Iron Oxygenases Derived from Actinomycetes in Rhodococcus Species for Investigation of Their Degradation Capabilities of Dibenzofuran and Chlorinated Dioxins
- A Hyperthermophilic Sulfur-reducing Archaebacterium, Thermococcus sp. DT1331,Isolated from a Deep-sea Hydrothermal Vent
- Cloning, Expression, and Characterization of a Minor Alkaline Protease from Bacillus sp. No. AH-101
- Substrate Specificity of Thermostable Alkaline Protease from Bacillus sp. No. AH-101
- Polychlorinated Biphenyl/Biphenyl Degrading Gene Clusters in Rhodococcus sp. K37, HA99, and TA431 Are Different from Well-Known bph Gene Clusters of Rhodococci