Molecular Cloning of β-isopropylmalate dehydrogenase Gene from Clostridium butyricum M588
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概要
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The gene for β-isopropylmalate dehydrogenase of Clostridium butyricum M588 has been cloned in E. coli. The genome of Clostridium butyricum M588 was digested with restriction endonuclease EcoRI and joined to plasmid pBR322. Competent E. coli HB101 cells were transformed with the hybrid plasmids and the leucine+ transformants were selected. The plasmid, pCE4, containing four EcoRI fragments, was isolated from the transformants. It was found that the 2.2kb EcoRI fragment on a reconstructed plasmid pCE1 contained the β-isopropylmalate dehydrogenase gene. Restriction analysis showed that the β-isopropylmalate dehydrogenase gene was located in the 1.4kb Bglll-EcoRl fragment and the BamRl site was inside the β-isopropylmalate dehydrogenase gene.
- 社団法人 日本農芸化学会の論文
著者
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KUDO TOSHIAKI
The Institute of Physical and Chemical Research(RIKEN)
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Kudo T
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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HONDA HIROSHI
The Institute of Physical and Chemical Research
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HORIKOSHI KOKI
The Institute of Physical and Chemical Research
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ISHII Kenji
The Institute of Physical and Chemical Research
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KUDO Toshiaki
The Institute of Physical and Chemical Research
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