Purification and Properties of 5'-Nucleotidase from Alkalophilic Bacillus No. C-3
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概要
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5'-Nucleotidase (EC 3. 1. 3. 5) from alkalophilic Bacillus no. C-3 was purifed to homogeneity. The molecular weight of the enzyme was 80,000 by gel filtration. The optimum pH for the activity was 9.5,and the enzyme was stable at pH 9.5-10.5 in a buffer containing 10 mM 2-mercaptoethanol. Substrate specificity study revealed that the enzyme acted on 5'-AMP strongly, on several 5'-nucleotides and ADP to a certain extent, but not on 3'-nucleotides, 2'-nucleotides, p-nitrophenyl phosphate, or ATP. The K_m value for 5'-AMP was 3.0×10^<-4> M. The enzyme required no divalent cation for its activity. The enzyme was inhibited by borate and arsenite ions but not by 1 mM EDTA.
- 公益社団法人日本生物工学会の論文
- 1989-03-25
著者
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HORIKOSHI KOKI
The Institute of Physical and Chemical Research
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IKURA YOKO
The Institute of Physical and Chemical Research
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