Manganese Dependent Production of 5'-Nucleotidase by Alkalophilic Bacillus No. C-3
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概要
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Alkalophilic Bacillus no. C-3 isolated from soil pruduced 5'-nucleotidase(EC-3.1.3.5)extracellularly when cultured in a medium containing Mn^<2+>. The unique point of enzyme peoduction is that the enzyme was produced well in the medium containing a rather high concentration of Mn^<2+>, in spite of a small difference in growth.The optimum concentration of Mn^<2+> for the enzyme peoduction was 10mM and over. Mn^<2+> could not be replaced by other divalent cations when added singly. In the presence of 10 mM Mn^<2+>, the enzyme production was repressed by the addition of 0.5 mM phosphate to the medium. The course of the enzyme peoduction closely paralleled the increase in growth. The optimum pH for the enzyme activity was 9.2-9.2,and KHCO_3-K_2CO_3 buffer was suitable for the enzyme.
- 公益社団法人日本生物工学会の論文
- 1989-02-25
著者
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HORIKOSHI KOKI
The Institute of Physical and Chemical Research
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IKURA YOKO
The Institute of Physical and Chemical Research
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Ikura Y
Research Development Corporation Of Japan And The Institute Of Physical And Chemical Research (riken
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Horikoshi Koki
The Institute Of Psysical And Chemical Research
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