Isolation of the Fragments from Alkalophilic Bacillus sp. DNA Which are Controlled by Temperature, pH or NaCl Concentration in pGR71 CAT Expression System
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概要
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The alkalophilic Bacillus DNA bank, cloned in the expression probe plasmid pGR71, has been screened for the p esence of genetic elements regulated by temperature, pH or NaCl concentration. It was found that several kinds of regulatory elements were obtained whose expression was controlled by temperature, pH or NaCl concentration. Temperature effect was analyzed further. In B. subtilis the 1000 base pair HindIII fragment (pGR71-11-73) of alkalophilic Bacillus sp. was shown to decrease CAT activity at higher temperatures. However, CAT activity of B. subtilis carrying the plasmid pGR71-13-36 (1.3 kb HindIII fragment) was not changed by temperature.
- 社団法人 日本農芸化学会の論文
著者
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KUDO TOSHIAKI
The Institute of Physical and Chemical Research(RIKEN)
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Kudo T
Inst. Physical And Chemical Res.(riken) Saitama Jpn
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HORIKOSHI KOKI
The Institute of Physical and Chemical Research
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YOSHITAKE Juichi
The Institute of Physical and Chemical Research
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USAMI Ron
The Institute of Physical and Chemical Research
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KUDO Toshiaki
The Institute of Physical and Chemical Research
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