Enzymatic Hydrolysis of Fats at High Substrate Concentrations in Biphasic Organic-Aqueous Systems
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概要
- 論文の詳細を見る
The hydrolysis of palm oil and beef tallow by lipase has been studied for practical applications in a biphasic isooctane-aqueous system using a high substrate concentration. The effective lipase concentration for the hydrolysis was found to be about 120 IU per g of substrate. The addition of twenty percent isooctane brought about the most rapid reaction and produced the highest percentage of hydrolysis. For both palm oil and beef tallow, a percentage of hydrolysis higher than 98% was achieved in the 20% isooctane system at a higher concentration of 50%. However, when the substrate concentration was higher than 50%, the final value of hydrolysis decreased as the concentration of the substrate increased. Utilization of recycled lipase was attempted using an ultrafiltration membrane reactor. Approximately 60% of the lipase activity was recorverable after each reaction.
- 社団法人日本生物工学会の論文
- 1987-02-25
著者
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SATO Seigo
Institute of Applied Biochemistry, University of Tsukuba
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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TAKAHASHI Joji
Institute of Applied Biochemistry, University of Tsukuba
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KOBAYASHI Tetsuo
Institute of Earth Sciences, Faculty of Science, Kagoshima University
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Kobayashi Tetsuo
Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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Kobayashi T
Univ. Tsukuba Ibaraki Jpn
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Kobayashi T
Laboratory Of Microbiology The Institute Of Physical And Chemical Research (riken)
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Kobayashi T
Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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Kobayashi Tetsuo
Institute Of Earth Sciences Faculty Of Science Kagoshima University
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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