Effects of Agitation on Enzymatic Hydrolysis of Cellulose in a Stirred-Tank Reactor
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概要
- 論文の詳細を見る
To find the best operating conditions for the effective enzymatic hydrolysis of cellulose materials using a stirred-tank reactor, the hydrolysis of two types of cellulose sources, Avicel(powder)and paper pulp(filament), was carried out under various agitator speeds.In ordinary operating conditions with a gas-liquid interface, the hydrolysis of the cellulose was facilitated by agitation, but excessive agitation caused a considerable reducation of the hydrolysis rate due to inactivation of cellulase, irrespective of the concentration and shape of cellulose materials. On the other hand, the reducation in the hydrolysis rate by agitation in the absence of a gas-liquid interface was far less than that in the presence of an interface. It was found that the lowering of cellulolytic activity of cellulase in the presence of substrate was larger than that in substrate-free systems. The reducation of cellulase activity by agitation was primarily due to the inactivation of two components, exo-β-1,4-glucanase and β-glucosidase, of the three major cellulase components.
- 公益社団法人日本生物工学会の論文
- 1983-12-25
著者
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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Tada Masazumi
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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