Utilization of Powdered Pig Bone as a Support for Immobilization of Lipase
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概要
- 論文の詳細を見る
Pig bone was examined for its suitability as a support material for lipase immobilization. It was observed that pig bone (PB) particles dispersed readily in both polar and nonpolar solvents, and lipase was easily adsorbed. In particular lipase adsorbed on olive oil-soaked pig bone (OPB) particles exhibited a higher hydrolytic activity than that in lipqse adsorbed on a selection of other representative supports, regardless of removing the prosoaked olive oil from the particles after immobilization of lipase. The optimum pH and temperature for hydrolytic activiey of OPB-adsorbed lipase were the same as those for free lipase, although thermal resistnace was increased by immobilization. When OPB-adsorbed lipase was used for repeated batch reactions of olive oil hydrolysis, an activity of more than 80% of the initial activity of each run could be retained after 46 h reaction. The results suggest that PB is an excellent support material.
- 公益社団法人日本生物工学会の論文
- 1989-05-25
著者
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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Negishi Satoshi
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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NEGISHI SATOSHI
Institute of Applied Biochemistry, University of Tsukuba
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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