Substrate Presoaking Effect on Immobilization of Protease on Pig Bone Particles
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概要
- 論文の詳細を見る
When pig bone (PB) particles and other support materials were presoaked in casein solution and crosslinked with glutaraldehyde, the activity of immobilized protease, was two or more times greater than those of the proteases immobilized on non-treated supports. Chitopearl was an exception. The highest activity, 25000 U/g-support, was obtained with the presoaked PB particles. The effect of presoaking the supports in the substrate solution on the immobilized protease was also abserved with other protein substrates. The effect of the presoaking treatment increased with an increase in the molecular weight of the protein. The increase in the activity of the immobilized enzyme was due to increases in both the amount of adsorbed protease and specific activity. Furthermore, the stability of protease immobilized on PB was remarkably improved by transforming the PB particles into a sheet.
- 公益社団法人日本生物工学会の論文
- 1990-11-25
著者
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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Negishi Satoshi
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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MUKATAKA SUKEKUNI
Insitute of Applied Biochemistry, University of Tsukuba
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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