Pressurized Culture of Escherichia coli for a High Concentration(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The cultural conditions for obtaining a high concentration of Escherichia coli K12 cells were investigated. A synthetic medium containing high concentrations of mineral salts was designed so as to support a high rate of cellular growth and the accumulation of a high cell concentration, without any additional feeding of inorganic nutrients, but with feeding of solid glucose. The pasteurization of the above mentioned medium at 65℃ was found to be advantageous for the cellular growth since usual sterilization at 121℃ caused denaturation of the medium, which was inhibitory for cellular growth. The accumulation of acetate, a well-known inhibitory product for the growth of E. coli, increased as the dissolved oxygen (DO) concentration decreased. When the DO concentration was maintained at the optimum value of 6.5ppm throughout the cultivation by pressurizing the culture system with oxygen gas, 134g (dry basis)/I of cells was accumulated in 12hr. The above culture conditions were applied to the cultivation of a recombinant E. coli strain transformed with pBR322trpAB, and 102g/l of recombinant cells was obtained with a stably maintained recombinant plasmid in 9hr under nonselective pressure.
- 社団法人日本農芸化学会の論文
- 1989-08-23
著者
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Matsui Toru
Institute Of Applied Biochemistry University Of Tsukuba
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Matsui T
Institute Of Applied Biochemistry University Of Tsukuba
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SATO Seigo
Institute of Applied Biochemistry, University of Tsukuba
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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TAKAHASHI Joji
Institute of Applied Biochemistry, University of Tsukuba
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YOKOTA Haruto
Institute of Applied Biochemistry, University of Tsukuba
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Matsui T
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Institute Of Applied Biochemistry University Of Tsukuba
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Yokota Haruto
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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