Purification and Properties of an Aminopeptidase from Mullet, Mugil cephalus, Roe(Biological Chemistry)
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概要
- 論文の詳細を見る
An aminopeptidase was purified from an aqueous extract of mullet roe in the presence of 2-mercaptoethanol by fractionation with ammonium sulfate and column chromatography on DEAE-cellulose and Sephadex G-200. The molecular weight of the enzyme was 184,000 by gel filtration, and the enzyme appeared to consist of two homogenous subunits. The optimal pH and optimal temperature for activity were 7.4 and 45℃, respectively. Puromycin, p-chloromercuribenzoic acid, and o-phenanthroline inhibited the enzyme non-competitively (their Ki=1.34μM, 0.113mM and 0.145 mM, respectively), while 2-mercaptoethylamine was competitive (Ki=0.056 mM). The enzyme was also inhibited by L-amino acids, in particular glutamic acid. The enzyme could hydrolyze a variety of α-aminoacyl β-naphthylamides and was most active on L-alanyl-β-naphthylamide. Judging from these properties, the mullet roe aminopeptidase resembles soluble alanyl aminopeptidase [EC 3.4.11.14].
- 社団法人日本農芸化学会の論文
- 1988-01-23
著者
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Matsui Toru
Institute Of Applied Biochemistry University Of Tsukuba
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KONOSU Shoji
Laboratory of Marine Biochemistry, Faculty of Agriculture, University of Tokyo
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MATSUI Takashi
Laboratory of Exercise Biochemistry, University of Tsukuba Graduate School of Comprehensive Human Sc
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Konosu Shoji
Laboratory Of Marine Biochemistry Faculty Of Agriculture The University Of Tokyo
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Matsui T
Institute Of Applied Biochemistry University Of Tsukuba
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CHIOU Tze-Kuei
Department of Food Science, National Taiwan Ocean University
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Chiou T‐k
National Taiwan Ocean Univ. Keelung Twn
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Chiou Tze-kuei
Affiliation: Department Of Food Science National Taiwan Ocean University
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Chiou Tze-kuei
Laboratory Of Marine Biochemistry Faculty Of Agriculture The University Of Tokyo
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Matsui T
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Matsui Takashi
Laboratory Of Exercise Biochemistry University Of Tsukuba Graduate School Of Comprehensive Human Sci
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Matsui Takashi
Laboratory Of Exercise Biochemistry And Neuroendocrinology University Of Tsukuba Graduate School Of Comprehensive Human Sciences
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Matsui Takashi
Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science
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