Effects of Nutritional Conditions on Plasmid Stability and Production of Tryptophan Synthase by a Recombinant Escherichia coli(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Effects of nutritional conditions and insertion direction of the tryptophan synthase (TSase) gene into a plasmid vector on the plasmid stability and the production of TSase in high cell concentration cultures were examined using recombinant Eseherichia coil (E. coli K12 IFO 3301) harboring pBR322trpAB(1) (the TSase gene was inserted at the EcoRI site of pBR322 in the clockwise direction) and pBR322trpAB(2) (counterclockwise direction). As to the effects of the insertion direction, the cells harboring pBR322trpAB(2) were slightly lower in the growth rate and the plasmid stability than those harboring pBR322trpAB(1). However, the former was higher in the productivity of TSase and the final cell concentration attained than the latter. On the other hand, the addition of organic nutrients, especially yeast extract, to TK-25 medium was very effective to improve the plasmid stability. Among the components of yeast extract, L-glutamic acid was found to be effective to improve both the plasmid stability and the production of TSase. When 1g/l of L-glutamic acid was added to TK-25 medium, a mineral synthetic medium developed for a high concentration culture, 115g (dry basis)/l of recombinant cells were obtained in 14 hr and the expression of TSase was maintained at 240-300U/mg-protein during the cultivation.
- 社団法人日本農芸化学会の論文
- 1990-03-23
著者
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Matsui Toru
Institute Of Applied Biochemistry University Of Tsukuba
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Matsui T
Institute Of Applied Biochemistry University Of Tsukuba
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SATO Hiroaki
Institute of Applied Biochemistry, University of Tsukuba
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SATO Seigo
Institute of Applied Biochemistry, University of Tsukuba
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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TAKAHASHI Joji
Institute of Applied Biochemistry, University of Tsukuba
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Matsui T
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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Sato Hiroaki
Institute For Environmental Management Technology National Institute Of Advanced Industrial Science
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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