Measurement of Hydrolytic Activity of Lipase by Isooctane Addition System(Analytical Chemistry)
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概要
- 論文の詳細を見る
An isooctane-addition agitated method is newly proposed for the assay of the hydrolytic activity of lipase. The hydrolytic activities of six kinds of microbial lipase were measured with this method at a high lipase concentration range and using olive oil, palm oil, and beef tallow as substrates. The newly proposed method gave equivalent or higher values than the previously proposed methods. In addition, the activity values calculated from the initial slopes of the progress curves gave a good proportional relation between enzyme concentration and activity in a wide range, although those calculated from the data obtained only at a fixed time (20min) were out of proportion to the enzyme concentration by all methods tested.
- 社団法人日本農芸化学会の論文
- 1989-09-23
著者
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KATAOKA HIROSHI
Institute of Applied Biochemistry, University of Tsukuba
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MUKATAKA Sukekuni
Institute of Applied Biochemistry, University of Tsukuba
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Kataoka Hiroshi
Institute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Insitute Of Applied Biochemistry University Of Tsukuba
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Sato Seigo
Institute Of Applied Biochemistry University Of Tsukuba
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Hayashi Kanako
Institute Of Symbiotic Science And Technology Tokyo University Of Agriculture And Technology
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Takahashi Joji
Insitute Of Applied Biochemistry University Of Tsukuba
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Takahashi J
Institute Of Applied Biochemistry University Of Tsukuba
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Takahashi Joji
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Insitute Of Applied Biochemistry University Of Tsukuba
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Mukataka S
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tskuba
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Mukataka Sukekuni
Institute Of Applied Biochemistry University Of Tsukuba
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