Production of Micro-Gel Beads by a Rotating Disk Atomizer

概要

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Micro-gel beads (200-1,200 μm in diameter) were produced by atomization of sodium alginate solution or a sodium alginate-perfluorocarbon mixture with a rotating disk. At any rotation speed of the disk (N) and low volumetric flow rate of the alginate solution (Q), dircet drop formation was observed at the edge of the disk. However, with increases in the volumetric flow rate, there was a transition from the direct drop formation to a ligament type of disintegration. The length of the ligaments increased with an increase in Q. Although the diameter of the micro-gel beads showed a bimodal distribution with the number of statellite beads increasing with an increase in Q and a decrease in N, the volume percentage of the satellite beads was negligible. The mean volume diameter for the micro-gel beads (d_v) was represented by a dimensionless equation : d_v/D=22.4N_<We>^<-0.43> N_<Re>^<0.08>N_Z^<0.07>, where D is the diameter of the diameter of the disk, N_<We>, N_<Re> and N_Z are the Weber, Reynolds, and Ohnesorge numbers, respectively. By immobilizing viable baker's yeast cells in micro-gel beads 500μm in diameter, the effectiveness factor for oxygen consumption at a DO concentration of 0.022mol/m^3 was 6 times higher, while the apparent K_m value was 50% lower, than when they were immobilized in gel beads 3.2mm in diameter.
社団法人日本生物工学会の論文
1989-07-25