High Renin productivity of rCHO Cells cultivated in Radial-Flow Nonwoven Fabric Mat Packed-Bed Reactor with Increasing Circulating Flow Rate
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概要
- 論文の詳細を見る
Radial-flow bioreactors are effective for reducing concentration gradients, because the flow area is large and the path for circulating culture broth is short. In this sutdy, a polyester nonwoven fabric mat was employed as a packing material in a radial-flow reactor, and the renin productio by recombinant Chinese hamster ovary (rCHO) cells in the bioreactor was investigated. The renin productivity of the cells was enhanced by use of an increasing circulating flow rate in the radial-flow reactor. It was also observed that the cells in the case of the increasing circulating flow rate formed aggregates, and the specific renin production rate was 6 times higher than that in the case of a constant circulating flow rate. This may imply that the cell aggregation which occured on the nonwovem fabric mat in the case of the increasing circulating flow rate may have been due in part to the enhancement of the renin productivity.
- 社団法人日本生物工学会の論文
- 1997-05-25
著者
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Wang P‐c
Institute Of Applied Microbiology University Of Tokyo
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Wang Pi-chao
Institute Of Applied Biochemistry University Of Tsukuba
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Wang P‐c
Graduate School Of Life And Environmental Science University Of Tsukuba
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Wang Pi-chao
Graduate School Of Life And Environmental Science University Of Tsukuba
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Wang Pi-chao
Institute Of Applied Biochemistry Tsukuba University
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MATSUMURA Masatoshi
Institute of Applied Biochemistry, University of Tsukuba
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KATAOKA HIROSHI
Institute of Applied Biochemistry, University of Tsukuba
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Kataoka Hiroshi
Institute Of Applied Biochemistry University Of Tsukada
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Matsuo S
Kyushu Univ. Fukuoka Jpn
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Matsumura M
Graduate School Of Life And Environmental Science University Of Tsukuba
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Matsumura Masatoshi
Institute Of Applied Biochemistry University Of Tsukuba
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Matsumura M
Graduate School Of Agriculture University Of Tsukuba
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Matsumura Masatoshi
Agricultual Sciences University Of Tsukuba
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MOTOBU Maki
National Institute of Animal Health
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MOTOBU MAKI
Institute of Applied Biochemistry, University of Tsukuba
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MATSUO SHIGERU
Fuji Photo Film Co. Ltd.
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Matsumura Masatoshi
Institute Applied Biochemisry Tsukuba University
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