Effect of Shear Stress on Recombinant Chinese Hamster Ovary Cells
スポンサーリンク
概要
- 論文の詳細を見る
In a previous study, we speculated that shear stress might enhance the renin productivity of recombinant Chinese hamster ovary(rCHO)cells cultivated ill a radial-flow reactor(Motobu et al. , J. Ferment. Bioeng. , 83, 443-450, 1997). In order to clarify the effect of shear stress on cell function, we designed a shear stress apparatus and investigated the cell morphology, cell cycle, renin productivity, and mRNA expression. In the case of subconfluent cells, which were mostly observed in the radial-how reactor, it was found that after 24 h of shear stress exposure at 0.02 and 0.082 N/m^2, the cell morphology became longer and slimmer, and cell growth in the subconfluent monolayer was inhibited as compared to that in a static culture. The results also showed that the cells were arrested in the G_o/G_1 phase of the cell cycle. However, renin productivity was found not to change under shear stress at 0.02 and 0.082 N/m^2 within 24 h, although the expression of renin mRNA under the shear stress condition was increased. With regard to confluent cells, it was surprising to find that shear stress did not influence the cell growth, cell cycle, renin productivity, or expression of mRNA within 24 h. Shear stress thus appears to affect subconfluent cells rather than confluent ones.
- 社団法人日本生物工学会の論文
- 1998-02-25
著者
-
Wang P‐c
Institute Of Applied Microbiology University Of Tokyo
-
Wang Pi-chao
Institute Of Applied Biochemistry University Of Tsukuba
-
Wang P‐c
Graduate School Of Life And Environmental Science University Of Tsukuba
-
Wang Pi-chao
Graduate School Of Life And Environmental Science University Of Tsukuba
-
Wang Pi-chao
Institute Of Applied Biochemistry Tsukuba University
-
MATSUMURA Masatoshi
Institute of Applied Biochemistry, University of Tsukuba
-
Matsumura M
Graduate School Of Life And Environmental Science University Of Tsukuba
-
Matsumura Masatoshi
Institute Of Applied Biochemistry University Of Tsukuba
-
Matsumura M
Graduate School Of Agriculture University Of Tsukuba
-
Matsumura Masatoshi
Agricultual Sciences University Of Tsukuba
-
MOTOBU Maki
National Institute of Animal Health
-
MOTOBU MAKI
Institute of Applied Biochemistry, University of Tsukuba
-
Matsumura Masatoshi
Institute Applied Biochemisry Tsukuba University
関連論文
- Effects of Nutrient-Poor and Anaerobic Conditions on Maintenance of R100-1 and RSF2124 Plasmids in Model Populations of Escherichia coli
- A Stochastic Approach to the Kinetics of Conjugative Transfer of Bacterial Plasmids
- Stability of Plasmid-Bearing Microorganisms in Batch and Continuous Cultures
- Crystal Structure of Bacillus stearothermophilus α-Amylase : Possible Factors Determining the Thermostability
- Production of Micro-Gel Beads by a Rotating Disk Atomizer
- 524 Production of Micro-Gel Beads using a Rotating Disk Atomizer.
- Nitrogen Removal and N_2O Emission in a Full-Scale Domestic Wastewater Treatment Plant with Intermittent Aeration
- 湖沼底泥から溶出するマンガンの藍藻類Microcysitis aeruginosaの増殖に及ぼす影響
- 濃厚鉄塩溶液滴下法を用いた合併処理浄化槽のリン除去プロセスの開発
- Anionic C-Terminal Proregion of Nematode Antimicrobial Peptide Cecropin P4 Precursor Inhibits Antimicrobial Activity of the Mature Peptide
- River die-away testに基づいた自然水域中でのラン藻毒microcystin LRの生分解性評価
- Microbial Extracellular Glycolipid Induction of Differentiation and Inhibition of the Protein Kinase C Activity of Human Promyelocytic Leukemia Cell Line HL60
- On-Site Nitrate Removal of Groundwater by an Immobilized Psychrophilic Denitrifier Using Soluble Starch as a Carbon Source
- 機能性微小動物の組合せによるアオコ形成Microcystis属の捕食・分解特性
- Degradation of the toxic cyanobacterium Microcystis viridis using predaceous micro-animals combined with bacteria
- Production of Human Growth Hormone by Protein-Free Cultivation of Anchorage-Dependent Cells with Porous Cellulose Carrier
- Effect of Additives on Oxidatio Kinetics of Sodium Sulfite Solution
- 3種の藻類の増殖に及ぼす湖沼底泥からの溶出物質の影響
- 霞ヶ浦における密度流拡散装置を用いた大規模汚濁湖沼底質改善
- Reduced Lipopolysaccharide (LPS)-Induced Nitric Oxide Production in Peritoneal Macrophages and Inhibited LPS-Induced Lethal Shock in Mice by a Sugar Cane (Saccharum officinarum L.) Extract
- Effect of Shear Stress on Recombinant Chinese Hamster Ovary Cells
- High Renin productivity of rCHO Cells cultivated in Radial-Flow Nonwoven Fabric Mat Packed-Bed Reactor with Increasing Circulating Flow Rate
- High Density Culture of Propionibacterium freudenreichii Coupled with Propionic Acid Removal System with Activated Charcoal
- Repression of Mycelia Extension from a Support Carrier Containing Immobilized Growing Aspergillus oryzae
- Application of a Rotating Ceramic Membrane to Dence Cell Culture
- Simultaneous Ethanol Fermentation and Stripping Process Coupled with Recification
- Continuous Ethanol Fermentation of Concentrated Sugar Solutions Coupled with Membrane Distillation Using a PTFE Module
- 下水汚泥焼却プロセスからの温室効果ガスN2Oの放出特性
- High-Affinity Binding Proteins for N-Acetylchitooligosaccharide Elicitor in the Plasma Membranes from Wheat, Barley and Carrot Cells : Conserved Presence and Correlation with the Responsiveness to the Elicitor
- Molecular interactions of chitin oligosaccharide elicitor binding proteins on the plasma membrane from plant cells as probed by affinity labeling agents
- Characteristics of N_2O Emission and Nitrogen Removal at A DO Controlled Intermittent Aeration Activated Sludge Process
- Ethanol Stripping by Pervaporation Using Porous PTFE Membrane
- Multi-well filtration ELISA法、MPN法およびFISH法による活性汚泥中のアンモニア酸化細菌の定量比較
- 超音波を用いた新規な水域底質モニタリング装置の開発
- Performance of Oxygen Transfer in a New Gas Entraining Fermentor
- Gas Entrainment in a New Gas Entraining Fermentor
- Gas Absorption Characteristics of the Tank-Type Fermentor with a Mechanical Gas Entrainer
- Microwave Zeeman Effect of Ketene
- Anionic C-Terminal Proregion of Nematode Antimicrobial Peptide Cecropin P4 Precursor Inhibits Antimicrobial Activity of the Mature Peptide
- Reconstruction of Renal Glomerular Tissue Using Collagen Vitrigel Scaffold
- Dialysis Bioreactor with Radial-Flow Fixed Bed for Animal Cell Culture
- 221 An Integrated System of Alcohol Fermentation and Pervaporation Using Porous PTFE Membrane
- 330 Performance of the Teflon Tube Ethanol Stripper
- Improvement of Treatment Efficiency of Thermophilic Oxic Process for Highly Concentrated Lipid Wastes by Nutrient Supplementation
- Utilization of Dehydrated Sewage Sludge as an Alternative Nutrient to Stimulate Lipid Waste Degradation by the Thermophilic Oxic Process
- 酸素透過性の異なる2種類の固定化担体を用いた一定酸素条件のもとでの同時硝化-脱窒
- Production of Highly Concentrated Xylitol by Candida magnoliae under a Microaerobic Condition Maintained by Simple Fuzzy Control
- A Novel Cyanobacteriolytic Bacterium, Bacillus cereus, Isolated from a Eutrophic Lake
- Surface modification of poly(L-lactic acid) affects initial cell attachment, cell morphology, and cell growth
- 215 Stability of Plasmid-bearing Microorganisms in environment
- 331 Separation of Ethanol from Fermentation Broth by Using Gas Stripping and Adsorption with Coconut Shell Charcoal
- 485. CHEMICAL DECOLORIZATION OF MOLASSES WASTEWATER IN ALCOHOL DISTILLERIES
- Characteristics of Hydrogen Sulfide Removal by Thiobacillus thiooxidans KS1 Isolated from a Carrier-Packed Biological Deodorization System
- Flocculation of Melanoidins Induced by Inorganic Ions
- High-Rate Seawater Denitrification Utilizing a Macro-Porous Cellulose Carrier
- 緑茶および緑茶ポリフェノールを用いたエビ病原性細菌ビブリオ汚染水の処理
- Application of Pure Oxygen in a New Gas Entraining Fermentor
- Effect of High Oxygen Concentration on the Growth of a Ethanol Assimilating Yeast, Candida sp.
- Construction of glomerular epithelial cells in vitro for removal of immune complex
- Development of Bioreactors for Denitrification with Immobilized Cells
- Effect of Stimulators such as GLP-1, PACAP, and Nicotinamide on Glucose-stimulated Insulin Secretion from Porcine Pancreatic Endocrine Cells in Long-term Culture
- Nitrification Performance of Marine Nitrifiers Immobilized in Polyester-and Macro-Porous Cellulose Carriers
- Detection of Telomerase Activity in Tissues and Primary Cultured Lymphoid Cells of Penaeus japonicus