Gluconic Acid Production at High Concentrations by Aspergillus niger Immobilized on a Nonwoven Fabric

概要

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Batch production of gluconic acid in the presence of a high concentration of glucose was investigated using free and immobilized mycelia of Aspergillus niger LAM 2094 with the aim of achieving repeatable constant production. Accumulation of 300 g/l or gluconate with a productivity of 60 g/l・h was achievable by intermittent addition of powdered glucose using filamentous-form mycelia in the presence of 150 ppm dissolved oxygen. However, this productivity became unattainable after a few repetitions. The use of pellet-form mycelia, in place of filamentous ones, did not prove effective either. However, when the mycelia were immobilized on a nonwoven fabric, a sustained level (220 g/l) of cluconate production was reproducible. Immobilized mycelia grown in a gas phase (air or oxygen) had a much longer durability than mycelia grown in a liquid culture medium. The gluconate-producing activity of immobilized mycelia grown in the presence of oxygen was much higher than that of mycelia grown in air. At 150 ppm dissoved oxygen, 220 g/l of gluconate was repeatedly produced 14 times at a constant production rate in a period of about 1,000 h.
社団法人日本生物工学会の論文
1989-06-25