高溶存酸素濃度下におけるAspergillus nigerの生育およびグルコン酸生産

概要

論文の詳細を見る
Effects of dissolved oxygen (DO) concentration on the growth of and the production of gluconic acid of Aspergillus niger IAM 2094 were investigated.Cellular yields decreased with increasing DO concentration up to around 50 ppm where the mycelial growth was completely inhibited by oxygen. In contrast, the rate of gluconate production by washed mycelia increased with increasing DO concentration, and no inhibitory effect was observed even at the DO of 150 ppm. The mycelial activity for gluconate production was increased in the mycelia grown at higher DO concentration.The rate of gluconate production followed Michaelis-Menten kinetics with respect to DO concentration, and the calues of Vmax were increased in mycelia grown at higher DO levels, while the values of Km were constant through the mycelia grown at various levels.The optimum concentration of glucose for gluconate production by washed mycelia was 15%, and no gluconate was produced in the presence of 25%. The rate of gluconate production as high as 24 g-gluconate/g-mycelia・h was attained under the DO concentration of 150 ppm using washed mycelia grown at 36 ppm.
1987-11-25