n-ブタンからの菌体外タンパク質の生産に及ぼす薬剤の影響

概要

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The production of extracellular protein from n-butane by Pseudomonas butanovara sp. nov. was investigated. The addition of surface active agents to the culture system enhanced the extracellular accumulation of protein; the amount of extracellular protein was nearly tripled by the presence of 0.05% of Tween 80. Antibiotics such as penicillin, D-cycloserine, and bacitracin also stimulated the secretion of protein. The amount of extracellular protein reached 6.2g/l at the 80th hour of cultivation in a laboratory fermentor with addition of 5 units/ml of bacitracin at the 35th hour of cultivation. Furthermore, the extracellular accumulation of protein was doubled by pressurizing the culture system, by which the rate of oxygen transfer was much increased.
社団法人日本生物工学会の論文
1986-09-25