水-有機溶媒二相反応系における脂肪の酵素分解

概要

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Hydrolysis of beef tallow and palm and olive oil by a lipase from Candida cylindracea in biphasic organic-aqueous reaction systems was investigated. The addition of an appropriate organic solvent to the reaction system stimulated the hydrolysis of these fats by lipase strongly. Among the organic solvents tested, isooctane was found to be the most sutiable solvenet for this purpose. The proportion of isooctane and phosphate buffer in the reaction mixture was best at the ratio of 1 : 9. The agitation speed had to be increased with increases of the isooctane fractioin to obtain a sufficient reaction rate. An agitation speed above 500 rpm was sufficient for the reactions. Under the above conditions, the reaction rate in the biphasic isooctane-aqueous buffer system was greatly enhanced compared to that in the organic-solvent-free system, and the percentage of hydrolysis reached almost 100%. In the former system, the deactivation of lipase was less than in the latter system. After 24 h, the residual specific activity remained at about 75% in the former system, but it decreased to about 30% in the latter system.
社団法人日本生物工学会の論文
1985-09-25