20-Hydroxyecdysone Production in Ajuga Hairy Root Controlling Intracelluar Phosphate Content Based on Kinetic Model

概要

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After phosphate depletion in a batch culture medium, the growth rare of Ajuga hairy root decreased and the content of a secondary metabolite, 20-hydroxyecdysone (20-HE), increased. The 20-HE content in Ajuga hairy root increased when the intracellular phosphate content was kept less than 5 mg/g-dry weight. Indoleacetic acid (IAA) supplementation (0.1 mg/l) increased the growth rate of Ajuga hairy root due to an increase i the number of root apical meristems although it led to a decrease in 20-HE content. From the above results, the culture was divided into two phases : a growth phase where all nutrient components in the medium are present above the critical level, and a secondary metabolite production phase where intracellular phosphate content is kept less than 5 mg/g-dry weight. A kinetic model was constructed to simulate the intracellular phosphate content during the culture. The fed-batch culture in which 0.1 mg/l of IAA was supplemented only at the begining of the culture, the intracellular phosphate content was kept below 5mg/g-dry weight after 10 d, and the phosphate was eliminated from the medium after 20 d, was carried out in a turbine-blade reactor by controlling the medium condition according to the conductivity of the cultuer medium and the simulation data.The cultuer provided approximately 3-fold incrase in 20-HE content, compared to the fed-batch culture without phosphate control.
公益社団法人日本生物工学会の論文
1995-10-25