High Concentration Culture of Bacillus subtilis spo^- Mutant Strain Carrying a Recombinant Plasmid

概要

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A Bacillus subtilis spo^- mutant strain harboring a recombinant plasmid was cultured at a high cell density concentratino by using a cross-flow filtration system. The cell concentration obtained was 300 as an optical density at 570 nm, which was six times as high as that of a fed-batch culture without using the cross-flow filtration system. However, the specific activity of plasmid-encoded β-galactosidase decreased by filtration. To improve the specific activity, the carbon source was stepwisely changed from glucose to starch. By using a fed batch culture combined with cross-flow filtration and the stepped change of carbon source, the production of the plasmid-encoded enzyme was improved 3 times compared with that of the fed-batch culture.
社団法人日本生物工学会の論文
1992-03-25