Comparison of Micro-Filtration Hollow Fiber Bioreactors for Mammalian Cell Culture

概要

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The production of tissue plasminogen activator (tPA) in various micro-filtration hollow fiber (MFHF) bioreactors for mammalian cell culture was compared. Whent he surface area of the bioreactor was increased 3-fold, tPA production per m^2 decreased 30%. Changing the angle of the medium distributor at the inlet of the hollow fiber cartridge increased the tPA production per m^2 by 15%. tPA production erached a maximum at 20d, but then decreased gradually. In order to maintain the tPA production over a long period, a cartridge whose surface area was reduced by 20% by decerasing the number of hollow fibers was utillized. tPA was produced stably, and the tPA prodcution per m^2 was 238mg/d/m^2. Cumulative tPA production for 50d reached 19g, and was lamost equal to that with the previous cartridge. It was concluded that the MFHT bioreactor can be scaled up if a uniform and sufficient supply of oxygen is achieved and good physiological conditions for mammaian cells are maintained.
社団法人日本生物工学会の論文
1997-05-25