High Level Production of Human Proapo A-I by Fed-Batch Culture of Recombinant Escherichia coli

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概要

• 論文の詳細を見る

• Three Escherichia coli strains, two recA^- strains (DH1 and YK537) and one recA^+ strain (KS476) harboring human proapo A-I expression plasmid pUS(pAI)^9 were cultivated in fed-batch mode using a syntehtic medium and the amounts of human proapo A-I accumulation were compared under various cultivation conditions. In the expression plasmid, nine proapo A-I genes weretandemly ligated downstream of the tac promoter. Experimental results indicated that selection of the host strain and cultivation temperature was important. Among the three E. coli strains checked, strain DH1 yielded the most effective production of human proapo A-I at 30℃.

• 社団法人日本生物工学会の論文
• 1993-02-25

著者

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• KOBAYASHI TAKESHI

  (Present address) Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Morimoto Yuuki

  Biosciences Laboratory Research Center Mitsubishi Kasei Corporation

• Morimoto Yuuki

  Biosciences Laboratory Mitsubishi Chemical Co. Research And Development Division Yokohama Research C

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Kobayashi Takeshi

  Dept.of Chemical Engineering Nagoya University

• OHTA KUNIHIKO

  Biosciences Laboratory, Research Center, Mitsubishi Kasei Corporation

• SHIBUI TATSURO

  Biosciences Laboratory, Research Center, Mitsubishi Kasei Corporation

• Ohta Kunihiko

  Biosciences Laboratory Research Center Mitsubishi Kasei Corporation

• Shibui Tatsuro

  Biosciences Laboratory Research Center Mitsubishi Kasei Corporation

• Kobayashi Takeshi

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• Kobayashi Takeshi

  Department Of Biotechnology:faculty Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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