Effects of Dissolved Oxygen Concentration on Anchorage-Dependent Animal Cell Growth and Erythropoietin Production

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概要

• 論文の詳細を見る

• Effects of dissolved oxygen concentration (DO) on anchorage-dependent cell growth, cell metabolism, and erythropoietin (EPO) production were investigated in both serum free and serum containing media. Specific EPO production rate increased significantly with the increase in DO from low to high levels with both media. Culture with serum free medium was found to be more dependent on DO than that with serum containing medium. The specific rates of cell growth and EPO production with controlling DO at 5.0 mg/l were 3.5-and 2.5- folds higher, respectively, than those with controlling DO at 0.5 mg/l. However, in serum containing medium, the specific growth rate was hardly influenced by DO levels, whereas the specific EPO production rate was 1.4-fold repressed under low DO levels. Influences of accumulated lactate and ammonium ion were negligible in the DO-controlled cultivation.

• 社団法人日本生物工学会の論文
• 1994-10-25

著者

• WANG Jin

  Department of Clinical Hematology, Second Hospital of Xi'an Jiaotong University

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• Park Y

  Shizuoka Univ. Shizuoka Jpn

• Park Yong

  (present Address)department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Universi

• Park Yong

  Biochemical Eng. Lab. Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka Uni

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology School Of Engineering Naogya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• HATA MIKIKO

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Wang J

  Department Of Physics The University Of Hong Kong

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Wang Jin

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Wang Jin

  Laboratory Of Analytical Microbiology National Center For Biomedicine Analysis Institute Of Microbio

• Hata Mikiko

  Department Of Biotechnology Factuly Of Engineering Nagoya University

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Wang Jin

  Department Of Biological Sience And The Mervin Bovaird Center For Studies In Molecular Biology And B

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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