On-Off Regulation of the Tryptophan Promoter in Fed-Batch Culture

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概要

• 論文の詳細を見る

• Fundamental properties of the trp promoter were investigated in fed-batch culture using a recombinant containing the lacZ gene controlled by this promoter. In tryptophan-dedicient conditions, the amount of β-galactosidase accumulated in the cell was 10% of total cellular proteins. In the presence of the amino acid, it was repressed at a lower level, but considerable expression was observed in the later stages of cultivation. Although increasing concentration of tryptophan seemed to repress the promoter more completely, it strongly inhibited the bacterial growth. On-off regulation of the promoter was achieved by controlling the tryprophan level during fed-batch culture.

• 社団法人日本生物工学会の論文
• 1986-12-25

著者

• KOBAYASHI Takeshi

  Department of Electrical Engineering, Faculty of Engineering Science, Osaka University

• IIJIMA SHINJI

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Iijima Shinji

  Department Of Biotechnology Faculty Of Engineering Nagoya University

• Kawai Shuji

  Department Of Chemical Engineering Faculty Of Engineering Nagoya University : (present Office)kao Co

• MIZUTANI SATORU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Kobayashi Takeshi

  Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University

• Kobayashi T

  Dep. Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Kobayashi T

  Univ. Tsukuba Ibaraki Jpn

• Mizutani S

  Kirin Brewery Co. Ltd. Kanagawa Jpn

• Kobayashi Takeshi

  Department Of Biotechnology Graduate School Of Engineering Nagoya University

• KOBAYASHI TAKESHI

  Department of Biotechnology, Faculty of Engineering, Nagoya University

• IIJIMA Shinji

  Department of Agricultural Chemistry, The University of Tokyo

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