The β-1,3-Exoglucanase Gene exgA (exg1) of Aspergillus oryzae Is Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface
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概要
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The biological role of ExgA (Exg1), a secretory β-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA (exg1) gene were analyzed. The exgA disruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial growth rate and conidiation efficiency were observed for the exgA-overexpressing mutant than for the exgA disruptant when β-1,3-glucan was supplied as sole carbon source. On the other hand, no difference in phenotype was observed between them in the presence or absence of the inhibitors of cell wall β-glucan remodeling when grown with glucose. exgA Expression was induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression. These data suggest that ExgA plays a role in β-glucan utilization, but is not much involved in cell wall β-glucan remodeling.
- 社団法人 日本農芸化学会の論文
- 2007-04-23
著者
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Ohashi Shinichi
Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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Machida Masayuki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn
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SANO Motoaki
Genome Biotechnology Laboratory, Kanazawa Institute of Technology
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TAKAHASHI Tadashi
Noda Inst. Sci. Res.
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KOYAMA Yasuji
Noda Inst. Sci. Res.
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Sano Motoaki
Genome Biotechnology Laboratory Kanazawa Institute Of Technology
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ABE Keietsu
Laboratory of Enzymology, Graduate School of Agriculture, Tohoku University
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MIZUTANI Osamu
Laboratory of Enzymology, Graduate School of Agricultural Science, Tohoku University
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Ohashi-kunihiro Sumiko
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Machida Masayuki
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Tamano Koichi
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Abe Keietsu
Laboratory Of Enzymology Department Of Molecular And Cell Biology Graduate School Of Agricultural Sc
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Abe Keietsu
Laboratory Of Molecular Enzymology Division Of Life Science Graduate School Of Agricultural Science
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MIZUTANI Osamu
National Research Institute of Brewing
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SATOH Yuki
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn
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ISHII Tomoko
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn
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TERABAYASHI Yasunobu
Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Techn
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OHTAKI Shinsaku
Laboratory of Molecular Enzymology, Division of Life Science, Graduate School of Agricultural Scienc
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Ishii Tomoko
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Satoh Yuki
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Ohtaki Shinsaku
Laboratory Of Molecular Enzymology Division Of Life Science Graduate School Of Agricultural Science
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Machida Masayuki
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Terabayashi Yasunobu
Research Institute For Cell Engineering National Institute Of Advanced Industrial Science And Techno
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Terabayashi Yasunobu
Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a
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Mizutani Osamu
National Res. Inst. Of Brewing
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Koyama Yasuji
Noda Inst. For Scientific Res.
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Mizutani Osamu
Laboratory Of Bioindustrial Genomics Department Of Bioindustrial Informatics And Genomics Graduate S
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Takahashi Tadashi
Noda Inst. For Scientific Res.
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Takahashi Tadashi
Noda Institute For Scientific Research
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