Enzymatic Properties of the Recombinant Serine-Type Carboxypeptidase OcpC, Which Is Unique to Aspergillus oryzae
スポンサーリンク
概要
- 論文の詳細を見る
Gene AO090103000153 is unique to Aspergillus oryzae RIB40 and A. flavus NRRL3357, and is speculated to encode a serine-type carboxypeptidase. In this study, we purified and characterized a heterologously expressed gene product of AO090103000153. 5′-Rapid amplification of cDNA ends indicated that the translation start site of the gene is located 1,586 bp downstream of the translation start site predicted by the genome sequencing project. The gene, starting from the revised translation start codon, termed ocpC, was transcribed constantly in A. oryzae RIB40. Purified recombinant OcpC exhibited the enzymatic properties of a serine-type carboxypeptidase. This protease was stable at temperatures below 45 °C and a low pH, and had broad substrate specificity for N-acylpeptides, but it exhibited significantly lower specific activity and a lower kcat value for substrates than previously reported serine-type carboxypeptidases from A. oryzae.
- 社団法人 日本農芸化学会の論文
- 2011-04-23
著者
-
Ishida Hiroki
Gekkeikan Sake Co. Ltd.
-
MIZUNO Takeshi
School of Agriculture, Nagoya University
-
YAMAGATA Youhei
Department of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
-
Kusumoto K
National Food Research Institute
-
OKAMOTO Ayako
Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Techno
-
Morita Hiroto
Department Of Urology Nihon University School Of Medicine
-
Yamagata Y
Tohoku Univ. Sendai Jpn
-
Kuriyama Ken-ichi
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Takeuchi Michio
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Takeuchi Michio
Department Of Agriscience And Bioscience Tokyo University Of Agriculture And Technology
-
Yamagata Youhei
Dep. Of Applied Molecular Biology And Biochemistry Tokyo Univ. Of Agriculture And Technol.
-
Yamagata Youhei
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Yamagata Youhei
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
Amano Hitoshi
Gifu R&d Center Amano Enzyme Inc.
-
KUSUMOTO Kenichi
National Food Research Institute
-
Kusumoto Ken-ichi
National Food Research Institute
-
Maeda Hiroshi
Department Of Urology Kurashiki Central Hospital
-
ABO Haruka
Department of Applied Molecular Biology and Biochemistry, Tokyo University of Agriculture and Techno
-
AMANO Hitoshi
Amano Enzyme Inc.
-
Morita Hiroto
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Okamoto Ayako
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Abo Haruka
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Maeda Hiroshi
Department Of Experimental Pathology Cancer Institute
-
Maeda Hiroshi
Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol
-
Maeda Hiroshi
Department Of Advanced Materials Science And Engineering Graduate School Of Engineering Yamaguchi Un
関連論文
- A genetic study of the arabidopsis circadian clock with reference to the Timing of Cab Expression 1 (TOC1) gene
- Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
- 前立腺癌における血清Basic Fetoproteinの臨床的意義 : PAP ・γ-Sm・PSAとの比較検討
- Echolucent Carotid Plaques as a Feature in Patients With Acute Coronary Syndrome
- Inca bone in forensic autopsy : a report of two cases with a review of the literature
- Carbon Molecular Sieve Membrane Prepared from Phenolic Resin
- Chenges in resistive index following extracorporeal schock wave lithotripsy
- Urinary undiversion for pelvic actinomycosis:A long-term follow up
- Patient-reported quality of life after radical prostatectomy for prostate cancer
- Ultrasensitive Assay of Prostate-Specific Antigen for Early Detection of Residual Cancer after Radical Prostatectomy
- Value of the Free to Total Prostate Specific Antigen Ratio and Prostate Specific Antigen Density for Detecting Prostate Cancer in Japanese Patients
- Value of Systematic Transition Zone Biopsy in the Detection of Prostate Cancer
- Nerve-Sparing Retroperitoneal Lymph Node Dissection for Metastatic Testicular Cancer
- Interstitial Laser Coagulation for Benign Prostatic Hyperplasia : Clinical Results after 12-Month Follow-up
- Generation of Metastatic Variants of Eker Renal Carcinoma Cell Lines for Experimental Investigation of Renal Cancer Metastasis
- Comparison of the Safety and Usefulness of Two Lithotripsy Systems : Pulse Dye Laser System versus Alexandrite Laser System
- Systematic Synthesis of Sulfur-containing p-Nitrophenyl α-Maltopentaoside Derivatives for a Differential Assay of Human α-Amylases
- Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli
- Characterization of Aspergillus oryzae glycoside hydrolase family 43 β-xylosidase expressed in Escherichia coil(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Molecular Cloning of ocpO Encoding Carboxypeptidase O of Aspergillus oryzae IAM2640
- High-Throughput Genotyping of Filamentous Fungus Aspergillus oryzae Based on Colony Direct Polymerase Chain Reaction(METHODS)
- Nitric Oxide Generation from Hydroxyurea via Copper-catalyzed Peroxidation and Implications for Pharmacological Actions of Hydroxyurea
- Bradykinin Generation Triggered by Pseudomonas Proteases Facilitates Invasion of the Systemic Circulation by Pseudomonas aeruginosa
- Non-oxygenated, Substrate-free Hyperpotassemic Cardioplegic Solution Affords Safe Protection of the Ischemic Myocardium during Open Heart Surgery
- Functional Changes of Polyethylene Glycol-modified Serine Proteinase from Aspergillus sojae and Interaction with α_2-Macroglobulin(Biological Chemistry)
- Novel Family of Sensor Histidine Kinase Genes in Arabidonsis thaliana
- Two Types of Putative Nuclear Factors that Physically Interact with Histidine-Containing Phosphotransfer (Hpt) Domains, Signaling Mediators in Histo-Asp Phosphorelay, in Arabidopsis thaliana
- ISOLATION AND CHARACTERIZATION OF FACTORS THAT INTERACT WITH AHPs, PUTATIVE HPt SIGNALING MEDIATORS IN Arabidopsis thaliana
- HISTIDINE-CONTAINING PHOSPHOTRANSFER (HPt) SIGNAL TRANSDUCERS IMPLICATED IN His-to-Asp PHOSPHORELAY IN Arabidopsis
- TWO-COMPONENT SIGNALING FACTORS CARRYING HPt DOMAIN IN Arabidopsis thaliana
- TWO-COMPONENT REGULATORY FACTORS IN Arabidopsis thaliana
- Soluble and Bound Forms of Aminopeptidase in Hen's Egg Yolk(Biological Chemistry)
- Improved Nitric Oxide Detection Using 2,3-Diaminonaphthalene and Its Application to the Evaluation of Novel Nitric Oxide Synthase Inhibitors
- Development of a Fluorescent Indicator for the Bioimaging of Nitric Oxide
- OE-068 Comparison of Clinical Implication between the Echolucent Carotid Plaques and Plaque Score in Patients with Acute Coronary Syndrome.(Acute Coronary Syndrome, Basic/Clinical 3 (IHD) : OE9)(Oral Presentation (English))
- Relation between Mitral Annular Calcification and Severity of Carotid Arterosclerosis in Stroke Patients
- Taka-Amylase A in the Conidia of Aspergillus oryzae RIB40
- Analysis of Secreted Proteins during Conidial Germination of Aspergillus oryzae RIB40
- Functional Analysis of an Endo-1,6-β-D-glucanase Gene (neg-1) from Neurospora crassa
- Cloning and Expression of the Exo-β-D-1,3-glucanase Gene (exgS) from Aspergillus saitoi(Biochemistry & Molecular Biology)
- Cloning and Expression of an Endo-1,6-β-D-glucanase Gene (neg1) from Neurospora crassa(Biochemistry & Molecular Biology)
- His-Asp phosphorelay signaling in Arabidopsis thaliana : Occurence of pseudo-response regulators (APRRs)
- Roles of Arabidopsis PSEUDO-RESPONSE REGULATOR (PRR) genes in the opposite controls of flowering time and organ elongation under long-day and continuous light conditions
- Hydration enthalpy for synthetic and cation exchanged A-type zeolites with special reference to zeolite heat pump media
- High-Throughput Screening for Isolation of Enhanced Green Fluorescent Protein Expressing Transformants of Filamentous Fungus Using an Image Analyzer(METHODS)
- Characterization of the Complex between α_2-Macroglobulin and a Serine Proteinase from Bacillus natto(Biological Chemistry)
- Identification of N-terminal Autodigestion Target Site in Subtilisin ALP I
- Alkaline-Resistance Model of Subtilisin ALP I, a Novel Alkaline Subtilisin
- Nucleotide Sequence of the Subtilisin NAT Gene, aprN, of Bacillus subtilis (natto)
- A New High-mannose Type N-Linked Oligosaccharide from Aspergillus Carboxypeptidase
- Enhanced Vascular Permeability in Solid Tumor Involving Peroxynitrite and Matrix Metalloproteinases
- Early Phase Tumor Accumulation of Macromolecules : A Great Difference in Clearance Rate between Tumor and Normal Tissues
- Peak C-Reactive Protein Concentration Correlates With Left Ventricular Thrombus Formation Diagnosed by Contrast Echocardiographic Left Ventricular Opacification in Patients With a First Anterior Acute Myocardial Infarction
- RELATIONSHIP OF ACCESSORY PATHWAY CONDUCTION TO THE ATRIAL VULNERABILITY IN WOLFF-PARKINSON-WHITE SYNDROME: COMPARISON BEFORE AND AFTER CATHETER ABLATION
- USEFULNESS OF INTRACARDIAC ELECTROGRAM RECORDINGS FOR DIAGNOSIS OF SINUS NODE FUNCTIONS IN SSS PATIENTS AND FOR ABLATION OF WPW SYNDROME AND ARIOVENTRICULAR NODE REENTRY TACHYCARDIA
- Genomewide Characterization of the Light-Responsive and Clock-Controlled Output Pathways in Lotus japonicus with Special Emphasis of its Uniqueness
- The Circadian Clock Regulates the Photoperiodic Response of Hypocotyl Elongation through a Coincidence Mechanism in Arabidopsis thaliana
- Transcript Profiling of an Arabidopsis PSEUDO RESPONSE REGULATOR Arrhythmic Triple Mutant Reveals a Role for the Circadian Clock in Cold Stress Response
- Involvement of Arabidopsis Clock-Associated Pseudo-Response Regulators in Diurnal Oscillations of Gene Expression in the Presence of Environmental Time Cues
- Insight into Missing Genetic Links Between Two Evening-Expressed Pseudo-Response Regulator Genes TOC1 and PRR5 in the Circadian Clock-Controlled Circuitry in Arabidopsis thaliana
- synthetic Studies of an 18-Membered Antitumor Macrolide, Tedanolide.5. Stereoselective Synthesis of the C13-C23 Part via Condensation of Two Fragments, C13-C17 and C18-C21,by Taking Adventage of the 3,4-Dimethoxybenzyl Protecting Group
- Synthetic Studies of the 18-Membered Antitumor Macrolide, Tedanolide. 3. Stereocontrolled Synthesis of the C1-C12 Part via a Synthesis of the C1-C7 Fragment by a Mismatched but Efficient Sharpless Dihydroxylation and Its Coupling with the C8-C11 Fragment
- Synthetic Studies of 18-Membered Antitumor Macrolide, Tedanolide. 2. Stereoselective Synthesis of the C1-C7 Fragment via a Mismatched but Highly Efficient Sharpless Dihydroxylation
- Homologs of the Aflatoxin Biosynthetic Gene ver-1 in Strains of Aspergillus oryzae and Related Species
- Functional Analysis of Genes Encoding Putative Oxidoreductases in Aspergillus oryzae, Which Are Similar to Fungal Fructosyl-Amino Acid Oxidase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Tumor-selective Blood Flow Decrease Induced by an Angiotensin Converting Enzyme Inhibitor, Temocapril Hydrochloride
- Changes of Peripheral Blood Lymphocyte Subsets in a Long-Term Survivor with Advanced Renal Cell Carcinoma
- Cisplatin, Vincristine, Methotrexate, Peplomycin, Etoposide (COMPE) Therapy for Disseminated Germ Cell Testicular Tumors
- Locally-Confined Signet-Ring Cell Carcinoma of the Prostate: A Case Report of a Long-Term Survivor
- Study of Deposition Process in Modulated RF Silane Plasma ( Plasma Processing)
- A Small Subfamily of Arabidopsis RADIALIS-LIKE SANT/MYB Genes : A Link to HOOKLESS1-Mediated Signal Transduction during Early Morphogenesis
- Specificity of a New Metalloproteinase form Penicillium citrinum(Biological Chemistry)
- Characterization of NikA Histidine Kinase and Two Response Regulators with Special Reference to Osmotic Adaptation and Asexual Development in Aspergillus nidulans
- Expression of the Cytokinin-Induced Type-A Response Regulator Gene ARR9 Is Regulated by the Circadian Clock in Arabidopsis thaliana
- Characterization of Genetic Links between Two Clock-Associated Genes, GI and PRR5 in the Current Clock Model of Arabidopsis thaliana
- Characterization of bZip-Type Transcription Factor AtfA with Reference to Stress Responses of Conidia of Aspergillus nidulans
- The Common Function of a Novel Subfamily of B-Box Zinc Finger Proteins with Reference to Circadian-Associated Events in Arabidopsis thaliana
- The Function of the Clock-Associated Transcriptional Regulator CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana
- Multidrug Resistance Protein 2 Implicates Anticancer Drug-Resistance to Sorafenib
- Molecular Cloning of ocpO Encoding Carboxypeptidase O of Aspergillus oryzae IAM2640
- Monitoring Global Gene Expression of Proteases and Improvement of Human Lysozyme Production in the nptB Gene Disruptant of Aspergillus oryzae
- TWO-COMPONENT REGULATORY FACTORS IN Arabidopsis thaliana
- Alternative Processing of Proproteins in Aspergilli kexB Gene Disruptants under Hyperosmotic Conditions
- Production of Polygalacturonase by Recombinant Aspergillus oryzae in Solid-State Fermentation Using Potato Pulp
- Enzymatic Production of Glucosylxylose Using a Cellobiose Phosphorylase-Yeast Combined System
- Functional Characterization of HY5 Homolog Genes Involved in Early Light-Signaling in Physcomitrella patens
- PHYTOCHROME-INTERACTING FACTORS PIF4 and PIF5 Are Implicated in the Regulation of Hypocotyl Elongation in Response to Blue Light in Arabidopsis thaliana
- Classification of the Genes Involved in the Two-Component System of the Moss Physcomitrella patens
- Characterization of an Aspergillus oryzae Cysteinyl Dipeptidase Expressed in Escherichia coli
- Characterization of Two Genes Encoding Ferritin-Like Protein in Actinobacillus actinomycetemcomitans
- Nanomolar Quantification and Identification of Various Nitrosothiols by High Performance Liquid Chromatography Coupled with Flow Reactors of Metals and Griess Reagent
- Enzymatic Properties of the Recombinant Serine-Type Carboxypeptidase OcpC, Which Is Unique to Aspergillus oryzae
- Effects of the Gel Size on the Thermal Behavior of N-isopropylacrylamide Gels in Relation to the Volume Phase Transition
- A Bilayer Formed by Soluble Amphiphiles with Single Chain at the Mica-Solution Interface
- Functional Characterization of HY5 Homolog Genes Involved in Early Light-Signaling in Physcomitrella patens
- Light-Responsive Double B-Box Containing Transcription Factors Are Conserved in Physcomitrella patens
- PHYTOCHROME-INTERACTING FACTOR 4 and 5 (PIF4 and PIF5) Activate the Homeobox ATHB2 and Auxin-Inducible IAA29 Genes in the Coincidence Mechanism Underlying Photoperiodic Control of Plant Growth of Arabidopsis thaliana
- Characterization of Shade Avoidance Responses in Lotus japonicus
- Disruption and Overexpression of Acid Phosphatase Gene (aphA) from a Miso Koji Mold, Aspergillus oryzae KBN630, and Characterization of the Gene Product
- Comparison of Acid Phosphatase Gene Expression Profiles in Solid-State Rice and Soybean Cultures of an Aspergillus oryzae Strain with Low Acid Phosphatase Activity (KBN8048) : Implications for Miso Brewing