Development of a Fluorescent Indicator for the Bioimaging of Nitric Oxide
スポンサーリンク
概要
- 論文の詳細を見る
Nitric oxide (NO) has been reported to play various roles as a signal transmitter. However, detailed functions of NO have yet to be clarified. We have developed a fluorescent indicator for NO imaging in living cells. The N-nitrosation of newly designed and synthesized 4-((3-amino-2-naphthyl)aminomethyl)benzoic acid (DAN-1) by NO yielded the highly fluorescent triazole-form. The membrane permeable ester derivative of DAN-1 (DAN-1 EE) was applied to the imaging of NO produced in activated rat aortic smooth muscle cells. After DAN-1 EE has been loaded into cells, the ester bond is hydrolyzed by intracellular esterase, yielding original DAN-1 with less permeability. The fluorescence intensity of the cells loaded with DAN-1 EE increased according to NO production. The imaging method with fluorescent indicators will be significant for the functional clarification of NO in vivo.
- 公益社団法人日本薬学会の論文
- 1997-12-15
著者
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NAGANO Tetsuo
Graduate School of Pharmaceutical Sciences, University of Tokyo
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Hirata Yasunobu
東京大学 内科学
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Maeda Hiroshi
Department of Urology, Shinko Clinic
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桐野 豊
徳島文理大学:香川薬学部:神経科学研究所:生物物理学会四国支部:東京大学
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Hirata Y
Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo
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Kirino Yutaka
Graduate School Of Pharmaceutical Sciences University Of Tokyo
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AKAIKE Takaaki
Department of Microbiology Kumamoto University School of Medicine
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NAGOSHI Hiroshi
Second Department of Internal Medicine, Faculty of Medicine, the University of Tokyo
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KOJIMA Hirotatsu
Graduate School of Pharmaceutical Sciences
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SAKURAI Kuniko
Graduate School of Pharmaceutical Sciences
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KIKUCHI Kazuya
Graduate School of Pharmaceutical Sciences
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HIRATA Yasunobu
Second Department of Internal Medicine, Faculty of Medicine
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KAWAHARA Shigenori
Graduate School of Pharmaceutical Sciences, Graduate School of Medicine, the University of Tokyo
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Nakagawa Hidetoshi
Faculty Of Pharmaceutical Sciences Kumamoto University
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KOUTAKA Hitomi
Daiichi Pure Chemicals Co., Ltd.
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Kikuchi K
Graduate School Of Pharmaceutical Sciences The University Of Tokyo:presto Jst Corporation
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Kikuchi Kazuya
Graduate School Of Engi Neering Osaka University:immunology Frontier Research Center Osaka Universit
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Nagano Tetsuo
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Kojima H
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Sakurai K
Hokkaido Coll. Pharmacy Hokkaido Jpn
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Akaike Takaaki
Department Of Microbiology Graduate School Of Medical Sciences Kumamoto University
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Nakagawa H
Mukogawa Women's Univ. Nishinomiya Jpn
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Nagoshi Hiroshi
Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo
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Akaike T
Department Of Microbiology Graduate School Of Medical Sciences Kumamoto University
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Koutaka Hitomi
Daiichi Pure Chemicals Co. Ltd.
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Kawahara Shigenori
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Maeda Hiroshi
Department Of Urology Kurashiki Central Hospital
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Maeda Hiroshi
Department Of Experimental Pathology Cancer Institute
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Maeda Hiroshi
Department Of Advanced Materials Science And Engineering Graduate School Of Engineering Yamaguchi Un
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