DEVELOPMENT OF A FLUORESCENT INDICATOR FOR NITRIC OXIDE BASED ON THE FLUORESCEIN CHROMOPHORE
スポンサーリンク
概要
- 論文の詳細を見る
Endogenous nitric oxide (NO) appears to modulate many physiological and pathophysiolgoical processes. In order to obtain direct evidence for NO functions in vivo, we have developed 4,5-diaminofluorescein (DAF-2) as a novel fluorescent indicator for NO. Green-fluorescent triazolofluorescein formed by the reaction of NO and DAF-2 affords high sensitivity for NO (detection limit : 5 nM). Membrane-permeable DAF-2 diacetate (DAF-2DA) was loaded into activated rat aortic smooth muscle cells, where the ester bonds are hydrolyzed by intracellular esterase, generating DAF-2. The fluorescence in the cells increased in a NO concentration-dependent manner. This imaging method should be useful for studies of the dynamic biological actions of NO at the molecular level with fine temporal and spatial resolution.
- 公益社団法人日本薬学会の論文
- 1998-02-15
著者
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NAGANO Tetsuo
Graduate School of Pharmaceutical Sciences, University of Tokyo
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Hirata Yasunobu
東京大学 内科学
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桐野 豊
徳島文理大院・香川薬・生物物理:阪大院・医・細胞神経科学
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桐野 豊
徳島文理大学:香川薬学部:神経科学研究所:生物物理学会四国支部:東京大学
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Hirata Y
Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo
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Kirino Y
Graduate School Of Pharmaceutical Sciences University Of Tokyo
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Kirino Y
Univ. Tokyo Tokyo Jpn
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Kirino Yutaka
Faculty Of Pharmaceutical Sciences Kyushu University
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Kirino Yutaka
Graduate School Of Pharmaceutical Sciences University Of Tokyo
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NAGOSHI Hiroshi
Second Department of Internal Medicine, Faculty of Medicine, the University of Tokyo
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KOJIMA Hirotatsu
Graduate School of Pharmaceutical Sciences
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SAKURAI Kuniko
Graduate School of Pharmaceutical Sciences
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KIKUCHI Kazuya
Graduate School of Pharmaceutical Sciences
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HIRATA Yasunobu
Second Department of Internal Medicine, Faculty of Medicine
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KAWAHARA Shigenori
Graduate School of Pharmaceutical Sciences, Graduate School of Medicine, the University of Tokyo
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Nakagawa Hidetoshi
Faculty Of Pharmaceutical Sciences Kumamoto University
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KOUTAKA Hitomi
Daiichi Pure Chemicals Co., Ltd.
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Kikuchi K
Graduate School Of Pharmaceutical Sciences The University Of Tokyo:presto Jst Corporation
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Kikuchi Kazuya
Graduate School Of Engi Neering Osaka University:immunology Frontier Research Center Osaka Universit
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Nagano Tetsuo
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Kojima H
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Sakurai K
Hokkaido Coll. Pharmacy Hokkaido Jpn
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Nakagawa H
Mukogawa Women's Univ. Nishinomiya Jpn
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Nagoshi Hiroshi
Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo
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Koutaka Hitomi
Daiichi Pure Chemicals Co. Ltd.
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Kawahara Shigenori
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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