Improved Nitric Oxide Detection Using 2,3-Diaminonaphthalene and Its Application to the Evaluation of Novel Nitric Oxide Synthase Inhibitors

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概要

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• A specific and sensitive detection method for nitric oxide (NO) in living cells and tissue culture systems is required in the search for novel NO synthase (NOS) inhibitors. We have improved a fluorometric determination with 2,3-diaminonaphthalene (DAN) by the addition of 2-phenyl-4,4,5,5-tetramehylimidazoline-3-oxide-1-oxyl (PTIO) as an oxidant to form NO_2 from NO. This method is 3 times more sensitive than that without PTIO, and is suitable for examining the NOS-inhibitory activity of large numbers of test compounds using a 96-well micropolate reader. The improved method was applied to N-monomethyl-L-arginine (L-NMMA) as a known inhibitor and the derivatives of 2-phenyl-1,2-benzisoselenazol-3(2H)-one as teat compounds in order to investigate the effect of these compounds on NO production from activated rat aortic smooth muscle cells. The results obtained indicate that this method is suitable for the rapid assay of large numbers of test compounds.

• 公益社団法人日本薬学会の論文
• 1998-12-15

著者

• NAGANO Tetsuo

  Graduate School of Pharmaceutical Sciences, University of Tokyo

• Hirata Yasunobu

  東京大学 内科学

• Maeda Hiroshi

  Department of Urology, Shinko Clinic

• Hirata Y

  Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo

• 樋口 恒彦

  東京大学薬学部

• AKAIKE Takaaki

  Department of Microbiology Kumamoto University School of Medicine

• NAGOSHI Hiroshi

  Second Department of Internal Medicine, Faculty of Medicine, the University of Tokyo

• NAKATSUBO Naoki

  Graduate School of Pharmaceutical Sciences

• KOJIMA Hirotatsu

  Graduate School of Pharmaceutical Sciences

• SAKURAI Kuniko

  Graduate School of Pharmaceutical Sciences

• KIKUCHI Kazuya

  Graduate School of Pharmaceutical Sciences

• HIRATA Yasunobu

  Second Department of Internal Medicine, Faculty of Medicine

• URANO Yasuteru

  Graduate School of Pharmaceutical Sciences

• HIGUCHI Tsunehiko

  Graduate School of Pharmaceutical Sciences

• Nakagawa Hidetoshi

  Faculty Of Pharmaceutical Sciences Kumamoto University

• KOUTAKA Hitomi

  Daiichi Pure Chemicals Co., Ltd.

• Kikuchi K

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo:presto Jst Corporation

• Kikuchi Kazuya

  Graduate School Of Engi Neering Osaka University:immunology Frontier Research Center Osaka Universit

• Nagano Tetsuo

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Kojima H

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Sakurai K

  Hokkaido Coll. Pharmacy Hokkaido Jpn

• Akaike Takaaki

  Department Of Microbiology Graduate School Of Medical Sciences Kumamoto University

• Nakagawa H

  Mukogawa Women's Univ. Nishinomiya Jpn

• HIGUCHI Teruhiko

  Neuropsychiatry Department, Showa University Fujigaoka Hospital

• Nagoshi Hiroshi

  Second Department Of Internal Medicine Graduate School Of Medicine The University Of Tokyo

• Akaike T

  Department Of Microbiology Graduate School Of Medical Sciences Kumamoto University

• 樋口 恒彦

  Waseda Univ.

• Koutaka Hitomi

  Daiichi Pure Chemicals Co. Ltd.

• Nakagawa Hiroshi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Maeda Hiroshi

  Department Of Urology Kurashiki Central Hospital

• Maeda Hiroshi

  Department Of Experimental Pathology Cancer Institute

• Maeda Hiroshi

  Department Of Advanced Materials Science And Engineering Graduate School Of Engineering Yamaguchi Un

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