Thiopalmitic Acid-Mediated Fe(III)-Nitrilotriacetate Reduction an Lipid Peroxidation

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概要

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• The mechanism of the antioxidant action of thiopalmitic acid (SH-Pal) was examined in an in vitro system measuring ferric (Fe(III))-nitrilotriacetate (NTA)- and Fe(III)-NTA/ascorbic acid (AsA)-induced lipid peroxidation of rat liver phospholipid liposomes and microsomes. The extent of lipid peroxidation was determined by measuring thiobarbituric acid reactive substances (TBARS). SH-Pal and glutathione (GSH) scarcely stimulated the Fe(III)-NTA-induced lipid peroxidation in contrast with the mode of action, being similar to those produced by reducing-agent antioxidants such as cysteine and AsA. SH-Pal reduced iron similar to the action produced by AsA and cysteine, but not that of GSH under the same conditions. Also, the reduction of iron by SH-Pal did not exhibit a pH dependency. Similarly, microsomal lipid peroxidation and oxygen consumption induced by Fe(III)-NTA/AsA were inhibited by the addition of SH-Pal in a time and dose dependent fashion, but GSH and cysteine exhibited a lower protective action. Time course studies on TBARS formation and oxygen consumption indicated the ability of SH-Pal to inhibit initiation and propagation reactions. Moreover, the microsomal lipid peroxidation induced by Cumene hydroperoxide (CumOOH) was progressively suppressed by the addition of increasing amounts of SH-Pal.These findings suggest that the antioxidant action of SH-Pal is partly due to complete reduction of iron at a faster rate and inhibition of oxygen consumption during the progress of the peroxidation. Further, SH-Pal has a protective action against free radical damage by hydroperoxy radical.

• 公益社団法人日本薬学会の論文
• 1996-05-15

著者

• 樋口 恒彦

  東京大学薬学部

• Tanaka M

  Otsuka Pharmaceutical Co. Ltd. Tokushima Jpn

• Takada K

  Showa College Of Pharmaceutical Sciences

• TANAKA Mitsuo

  Showa College of Pharmaceutical Sciences

• TAKADA Kimihiko

  Showa College of Pharmaceutical Sciences

• HIGUCHI Toshiyuki

  Showa College of Pharmaceutical Sciences

• NAKAGAWA Mitsuo

  Showa College of Pharmaceutical Sciences

• HIGUCHI Teruhiko

  Neuropsychiatry Department, Showa University Fujigaoka Hospital

• Nakagawa M

  Showa College Of Pharmaceutical Sciences

• 樋口 恒彦

  Waseda Univ.

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