Optimum Conditions for the ^<13>C-Phenylalanine Breath Test

概要

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We have conducted optimizaiton studies to develop a superior ^<13>C-phenylalanine breath test for the diagnosis of liver disease. First, we examined the optimum ^<13>C-labeling position in phenylalanine for use in a breath test based on infrared spectroscopic detection of ^<13>CO_2 in exhaled air. L-[1-^<13>C]Phenylalanine gave the best result. Next, a suitable dosage to give a short peak time (the time expressed in minutes as which ^<13>CO_2 excretion is maximal) after administration was determined. The ^<13>CO_2/^<12>CO_2 ratio in exhaled air after administration of 100 mg/body of L-[1-^<13>C]phenylalanine peaked sharply at 15 min. We also examined the effect of food on the hepatic metabolism of L-[1-^<13>C]phenylalanine. We found that a fasting period of over 7 h before the test resulted in a higher ^<13>CO_2 peak excretion. The peak appeared sooner than that in the ^<13>C-phenacetin breath test and, therefore, the ^<13>C-phenylalanine breath test appears preferable for the rapid evaluation of hepatic function.
公益社団法人日本薬学会の論文
1998-08-15