Development of an Enzyme-Linked Immunosorbent Assay System to Determine the Presence of Antibodies Specific for Taxane Structures
スポンサーリンク
概要
- 論文の詳細を見る
Taxanes, which are widely used in treatment of numerous cancer types, are well-known to induce hypersensitivity reactions (HSR), especially in the case of paclitaxel. Although the cause of the HSR is commonly thought to be a non-immunological direct effect of the diluent which is used to dissolve paclitaxel, some reports suggest the possibility of the presence of an immunological reaction to the common taxane structure. The aim of this study was to establish a method to determine the presence of anti-taxane antibodies in body fluids of patients who have previously received paclitaxel, in order to estimate the risk of the occurrence of HSR to other taxane compounds, such as docetaxel. To prepare an enzyme-linked immunosorbent assay (ELISA) plate for determining taxanes, 10-deacetylbaccatin III (DAB) was first succinylated by use of dimethylaminopyridine and succinic anhydride in dried pyridine. After the succinylation reaction, three different products were obtained, and these were confirmed as 7-succinoyl DAB (7-DAB), 10-succinoyl DAB (10-DAB), and 7,10-disuccinoyl DAB (7,10-DAB) by 1H-NMR analysis. Each of these three products was conjugated with bovine serum albumin (BSA), and adsorbed on an ELISA plate. By using a commercially available anti-taxane monoclonal antibody as a model antibody, the detection limit of the anti-taxane antibodies on the 7-DAB-BSA-, 10-DAB-BSA-, and 7,10-DAB-BSA-conjugated ELISA plate was estimated as 0.3, 1 and 10 ng/ml, respectively. The ELISA system established in this study may therefore be useful for estimating the risk of HSR to taxanes in a patient prior to the use of these drugs.
著者
-
MATSUZAWA Yasuo
Department of Internal Medicine, Toho University Sakura Medical Center
-
Okuno Hiroaki
Department Of Orthopaedic Surgery Hyogo Medical College
-
Matsuzawa Yasuo
Department Of Internal Medicine Toho University School Of Medicine Sakura Hospital
-
Matsuzawa Yasuo
Department Of Internal Medicine Toho University Sakura Medical Center
-
Ishii Toshihiro
Department Of Pharmacy Faculty Of Medicine Sakura Hospital Toho University
-
Shirai Koji
The Center Of Diabetes Endocrinology And Metabolism Toho Univ. Sakura Medical Center
-
Yokoyama Yuusaku
Department Of Pharmaceutical Sciences Toho University
-
Muto Satoshi
Department Of Clinical Chemistry School Of Pharmaceutical Sciences Toho University
-
Kawashima Tatsuo
Department Of Internal Medicine Toho University School Of Medicine Sakura Hospital
-
Kouyama Yuichi
Department of Disease Analysis, Faculty of Pharmaceutical Sciences, Toho University
-
Masaka Toru
Department of Pharmacy, Toho University Sakura Medical Center
-
Masaka Toru
Department Of Pharmacy Sakura Medical Center Toho University
-
Okuno Hiroaki
Department Of Orthopaedic Surgery Hyogo College Of Medicine
-
Matsuzawa Yasuo
Department of Respiratory Medicine, Toho University Sakura Medical Center
-
Shirai Koji
The Center of Diabetes, Endocrinology, and Metabolism, Toho University Sakura Medical Center
-
Ishii Toshihiro
Department of Medicinal Chemistry, Meiji Pharmaceutical University
-
Ishii Toshihiro
Department of Pharmacy, Toho University Sakura Medical Center
-
Yokoyama Yuusaku
Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Toho University
-
Kawashima Tatsuo
Department of Respiratory Medicine, Toho University Sakura Medical Center
-
Okuno Hiroaki
Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Toho University
-
Muto Satoshi
Department of Disease Analysis, Faculty of Pharmaceutical Sciences, Toho University
関連論文
- Effects of Synthetic Sphingosine-1-Phosphate Analogs on Cytosolic Phospholipase A2α–Independent Release of Arachidonic Acid and Cell Toxicity in L929 Fibrosarcoma Cells: the Structure–Activity Relationship
- Activation of Cytosolic Phospholipase A_2α by Epidermal Growth Factor (EGF) and Phorbol Ester in HeLa Cells : Different Effects of Inhibitors for EGF Receptor, Protein Kinase C, Src, and C-Raf
- Effects of Synthetic Sphingosine-1-Phosphate Analogs on Cytosolic Phospholipase A_2α-Independent Release of Arachidonic Acid and Cell Toxicity in L929 Fibrosarcoma Cells : the Structure-Activity Relationship
- Activation of Cytosolic Phospholipase A2α by Epidermal Growth Factor (EGF) and Phorbol Ester in HeLa Cells: Different Effects of Inhibitors for EGF Receptor, Protein Kinase C, Src, and C-Raf
- Smoking Behaviors and Attitudes Among School Teachers in Mie, Japan
- Night-Shift Work Related Problems in Young Female Nurses
- Evaluation of an Interference Screw for Tendon Reattachment to Small Bones
- Role of Galectin-3 in Human Pulmonary Fibrosis
- Preparation of Liposomes Modified with Lipopeptides Using a Supercritical Carbon Dioxide Reverse-phase Evaporation Method
- Development of an Enzyme-Linked Immunosorbent Assay System to Determine the Presence of Antibodies Specific for Taxane Structures
- A frameshift variant of CYP2C8 was identified in a patient who suffered from rhabdomyolysis after administration of cerivastatin
- Targeted Proteo-Glycomics Analysis of Sialyl Lewis X Antigen Expressing Glycoproteins Secreted by Human Hepatoma Cell Line
- Determination of Relative and Absolute Stereochemistry of Cephalosporolide D and Its Enantioselective Total Synthesis
- Evaluation of ^C-Phenylalanine and ^C-Tyrosine Breath Tests for the Measurement of Hepatocyte Functional Capacity in Patients with Liver Cirrhosis
- Optimum Conditions for the ^C-Phenylalanine Breath Test
- Chemistry of Unprotected Amino Acids in Aqueous Solution : Direct Bromination of Aromatic Amino Acids with Bromoisocyanuric Acid Sodium Salt under Strong Acidic Condition
- Indomethacin Decreases Arachidonic Acid Uptake in HCA-7 Human Colon Cancer Cells
- Low-molecular Weight Amines Have Marked Potential to Promote Colitis in a Mouse Experimental Model: A Possible Proposal of in vivo Formation of Their Chloramines
- Mediastinal Lymphadenopathy without Cervical Lymphadenopathy in a Case of Kikuchi- Fujimoto Disease
- Expression of TNF-related Apoptosis-inducing Ligand (TRAIL) and TRAIL Receptor 1 on Cancer and Normal Tissues in Patients with Non-small Cell Lung Cancer
- Nitric Oxide and Immune Complexes Are Involved in the Induction of a Novel Luminol Chemiluminescencwe in Cytotoxic Macrophages
- Human Mannose-Binding Lectin Preferentially Binds to Human Colon Adenocarcinoma Cell Lines Expressing High Amount of Lewis A and Lewis B Antigens
- Correlation Between Cyclosporin A Concentration and Lipid Concentration in Patients with Ulcerative Colitis
- Posterior interosseous nerve palsy caused by synovial chondromatosis arising in the annular periradial recesses of the elbow
- Inhibitory effects of clinical reagents having anti-oxidative activity on transforming growth factor-β1-induced expression of α-smooth muscle actin in human fetal lung fibroblasts
- The Blood Concentration and Therapeutic Efficacy of Azathioprine in Patients Receiving Infliximab Combination Therapy for Refractory Crohn's Disease : A Clinical Study
- Assessment of Combined Therapy with Purine Derivatives and Infliximab in Patients with Refractory Crohn's Disease
- Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan : predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-produdng non-baumannii Acinetobacter species
- Successful Treatment of Fulminant Community-Acquired Pseudomonas aeruginosa Necrotizing Pneumonia in a Previously Healthy Young Man
- Impaired Consciousness Caused by a Metastatic Adrenal Tumor of Pulmonary Adenocarcinoma
- Genotyping of skin and soft tissue infection (SSTI)-associated methicillin-resistant Staphylococcus aureus (MRSA) strains among outpatients in a teaching hospital in Japan : application of a phage-open reading frame typing (POT) kit
- PREDICTION OF RECURRENCE FOR NON-SMALL CELL LUNG CANCER BY COMBINED ANALYSIS OF MOLECULAR MARKERS AND 18F 2-FLUORO-2-DEOXY-D-GLUCOSE POSITRON EMISSION TOMOGRAPHY