Preparation of Liposomes Modified with Lipopeptides Using a Supercritical Carbon Dioxide Reverse-phase Evaporation Method
スポンサーリンク
概要
- 論文の詳細を見る
Although liposomes are considered to be one of the most promising carriers for drug delivery systems (DDS), they have drawbacks such as insufficient drug-entrapment efficiency and long-term stability. The objectives of this study are to improve the trapping efficiency by addition of lipopeptides (LPs), and using a supercritical CO2 reverse-phase evaporation (SCRPE) process, along with incorporation of PEG-modified phospholipids to improve long-term stability. In this study, bovine serum albumin (BSA) was used as a model drug substance for entrapment by liposomes.Improvements in the entrapment efficiency and stability of liposomes were achieved by modification with LPs and use of a SCRPE preparation process. The BSA-entrapment efficiency of liposomes modified with cationic LPs with arginine residues, as a result of their ionic interactions, was six times that of liposomes prepared by the Bangham method. Use of a SCRPE method along with LP modification further enhanced entrapment and enabled spontaneous formation of unilamellar liposomes with long-term stability. Liposomes consisting of DPPC/Chol/C16-Arg2/DSPE-PEG2000 (60/30/5/5), with up to 70% entrapment efficiency for BSA and a stability level of 90% for over 40 h, were obtained. DSC and SAXS analyses indicated that certain amounts of LP in the DPPC induced phase-transitional and structural changes in the lamellar membrane, and these changes improved the DDS carrier properties.The SCRPE method provides organic-solvent-free liposomes, and the LPs for the liposome modification are derivatives of amino acids and fatty acids, which are sustainable and biocompatible materials. This study therefore suggests that there are opportunities for the development of novel DDS carriers with excellent performance and which address environmental concerns.
著者
-
Abe Masahiko
Department Of Internal Medicine Hokkaido Prefectural Haboro Hospital
-
Sakai Kenichi
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
-
Yagi Nobuhiro
Department Of Cardiovascular Medicine The University Of Tokyo Graduate School Of Medicine
-
Okuno Hiroaki
Department Of Orthopaedic Surgery Hyogo Medical College
-
Sakamoto Kazutami
Department Of Pure And Applied Chemistry Faculty Of Science And Technology Tokyo University Of Scien
-
Sakai Hideki
Department Of Nephro-urology Nagasaki University Graduate School Of Biomedical Sciences
-
YOKOYAMA Yuusaku
Department of Pharmaceutical Sciences, Toho University
-
Aburai Kenichi
Department of Pure and Applied Chemistry, Tokyo University of Science
-
Yokoyama Yuusaku
Department Of Pharmaceutical Sciences Toho University
-
Okuno Hiroaki
Department Of Orthopaedic Surgery Hyogo College Of Medicine
-
Okuno Hiroaki
Department of Pharmaceutical Sciences, Toho University
-
Sakai Hideki
Department of Pure and Applied Chemistry, Tokyo University of Science
-
Sakai Hideki
Department of Industrial Chemistry, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
-
Yagi Nobuhiro
Department of Pharmaceutical Sciences, Toho University
-
Sakamoto Kazutami
Department of Pure and Applied Chemistry, Tokyo University of Science
関連論文
- A Newly Estimated Glomerular Filtration Rate Is Independently Associated with Arterial Stiffness in Japanese Patients
- Rheological Behavior of Polyoxyethylene Phytosterol-Polyoxyethylene Cholesterol/Tetraethylene Glycol Monododecyl Ether/Water Systems
- Cethyltrimethylammonium Bromide-Mediated Hexagonal-Structured Self-Assembly of Nanocrystalline Titania : Gravity Effect on Framework Formation and Crystal Growth
- ジェミニ型界面活性剤を用いた壁膜に結晶構造を有するメソ構造化チタニアの室温合成
- Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer
- Gastrointestinal stromal tumor of the stomach treated by urgent laparoscopic partial gastrectomy in an elderly woman : Report of a case
- Syntheses of Cationic Surfactants having Two Ferrocenylalkyl Chains
- PE-379 Nanoparticle-mediated Systemic Delivery of siRNA Targeting KLF5 Inhibit Tumor Angiogenesis and Growth in Vivo(Genetics/Genetically engineered models/Gene therapy-2 (M) PE64,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of
- B-6 Coexpression of keratinocyte glowth factor (KGF) and its receptor (KGFR) correlates with poor prognosis in prostate cancer.
- Vacuum-Ultraviolet Circular Dichroism Spectrophotometer Using Synchrotron Radiation: Optical System and On-line Performance
- Adsorption of Actives in Ophthalmological Drugs for Over-The-Counter on Soft Contact Lens Surfaces
- Evaluation of an Interference Screw for Tendon Reattachment to Small Bones
- Clinical Evaluation of Serum Prostate-Specific Antigen-Alpha^1-Antichymotrypsin Complex Values in Diagnosis of Prostate Cancer: A Cooperative Study
- OJ-135 A Newly Estimated Glomerular Filtration Rate is an Independent Indicator of Arterial Stiffness(Atherosclerosis, clinical-4 (H) OJ23,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- Effectiveness of Brain Hypothermia Treatment in Patients With Severe Subarachnoid Hemorrhage : Comparisons at a Single Facility
- Endovascular Treatment of Asymptomatic Cerebral Aneurysms : Anatomic and Technical Factors Related to Ischemic Events and Coil Stabilization
- Novel Approach for the Preparation of Metal Containing Mesoporous Silica Using Solubilization of Fatty Acid Salt
- Solution Properties of Sulfate-Type Fluoro-Hybrid Anionic Surfactants with a Benzene Ring in Their Molecules
- A Narrow Bicontinuous Microemulsion Domain in Mixed Polymeric Silicone Systems
- Electrochemical Behavior of a Ferrocene-Modified Cationic Surfactant Mixed with an Anionic Surfactant in Aqueous Solutions
- Transesterification by (Hydroxo)nickel(II) Complex in the Presence of External Alcohol
- The Syntheses of (Chloro) Iron (II) Complexes by Using Tridentate Polypyridine Ligands
- Triple-Decker Sandwich Structure of a New Rubidium(I)Tetraimino Macrocyclic Complex
- Sysntheses and Structures of a Novel Trinaphthylene Hexaimino Cryptand:Three Different Conformations of a Macrobicyclic Ligand
- Conformational Change of a Macrobicyclic Complex. Structure of Free Ligand and Interactions with DMSO
- Synthesis and Characterization of Barium (II) Macrobicyclic Complexes and Related Compounds
- Unicentric Castleman's disease with leiomyosarcoma : A rare association
- PROLIFERATING CELL NUCLEAR ANTIGEN IN ACTH-INDEPENDENT BILATERAL MACRONODULAR ADRENAL HYPERPLASIA
- Microscopic Observation of Photocatalytic Reaction Using Microelectrode: Spatial Resolution for Reaction Products Distribution
- Selective Killing of a Single Cancerous T24 Cell with TiO2 Semiconducting Microelectrode under Irradiation
- Involvement of aquaporin-5 in differentiation of human gastric cancer cells
- Leukotrienes-Mediated Effects of Water Extracts from Sargassum horneri, a Marine Brown Alga, on Cl^- Absorption in Isolated Rat Colon
- Expression of ATP1AL1, a Non-Gastric Proton Pump, in Human Colorectum
- Is Rabbit CLCA1 Related to the Basolateral Ca^-Dependent Cl^- Channel of Gastric Parietal Cells?
- Mobilization of Intracellular Ca^ by Thromboxane A_2 Does Not Affect Ca^-Activated K^+ Channels in Rat Colonic Crypt Cells
- Prostaglandin E_2-Activated Housekeeping Cl^- Channels in the Basolateral Membrane of Rat Gastric Parietal Cells
- Preparation of Liposomes Modified with Lipopeptides Using a Supercritical Carbon Dioxide Reverse-phase Evaporation Method
- DSPC/DLPC Mixed Films Supported on Silica: A QCM-D and Friction Force Study
- Physicochemical Analysis of Liposome Membranes Consisting of Model Lipids in the Stratum Corneum
- Simulation of Dynamic Behavior of Surfactants on a Hydrophobic Surface Using Periodic-Shell Boundary Molecular Dynamics
- Recent Advances in Gemini Surfactants: Oleic Acid-Based Gemini Surfactants and Polymerizable Gemini Surfactants
- Development of an Enzyme-Linked Immunosorbent Assay System to Determine the Presence of Antibodies Specific for Taxane Structures
- Papillary Tumor of the Pineal Region
- Skeletal-related events in urological cancer patients with bone metastasis : A multicenter study in Japan
- Combined adrenal adenoma and myelolipoma in a patient with Cushing's syndrome : Case report and review of the literature
- Chemistry of Unprotected Amino Acids in Aqueous Solution : Direct Bromination of Aromatic Amino Acids with Bromoisocyanuric Acid Sodium Salt under Strong Acidic Condition
- Vacuum-Ultraviolet Circular Dichroism Spectrophotometer Using Synchrotron Radiation: Optical System and Off-line Performance
- Polymerizable Anionic Gemini Surfactants: Physicochemical Properties in Aqueous Solution and Polymerization Behavior
- Dielectric Relaxation Analysis of Single-Stranded DNA in Liquid Crystals
- SAXS and Rheometry Studies of Diglycerol Monolurate Reverse Micelles in Styrene
- Oleic Acid-Based Gemini Surfactants with Carboxylic Acid Headgroups
- Sulfonic-Hydroxyl-Type Heterogemini Surfactants Synthesized from Unsaturated Fatty Acids
- Adsorption of Cationic Monomeric and Gemini Surfactants on Montmorillonite and Adsolubilization of Vitamin E
- GTP-Binding Protein-Mediated Production of Superoxide Anion in Rabbit Gastric Parietal Cells
- Coexistence of Creutzfeldt-Jakob disease, Lewy body disease, and Alzheimer's disease pathology : An autopsy case showing typical clinical features of Creutzfeldt-Jakob disease
- Kainic acid may enhance hippocampal NO generation of awake rats in a seizure stage-related fashion
- Reversible Clinical and Magnetic Resonance Imaging of Central Pontine Myelinolysis Following Surgery for Craniopharyngioma : Serial Magnetic Resonance Imaging Studies
- Identification of Human P450 Isoforms Involved in the Metabolism of the Antiallergic Drug, Oxatomide, and Its Inhibitory Effect on Enzyme Activity (Miscellaneous)
- Endogenous Arachidonic Acid Inhibits Hypotonically-Activated Cl^- Channels in Isolated Rat Hepatocytes
- Function of K+–Cl− Cotransporters in the Acid Secretory Mechanism of Gastric Parietal Cells
- Identification of Human P450 Isoforms Involved in the Metabolism of the Antiallergic Drug, Oxatomide, and Its Kinetic Parameters and Inhibition Constants(Miscellaneous)
- Nonionic Surfactant Mixtures in an Imidazolium-Type Room-Temperature Ionic Liquid
- Catanionic Mixtures Forming Gemini-like Amphiphiles
- Hierarchic Template Approach for Synthesis of Silica Nanocapsules with Tuned Shell Thickness
- Posterior interosseous nerve palsy caused by synovial chondromatosis arising in the annular periradial recesses of the elbow
- Inhibition of ecto-ATPase activity by curcumin in hepatocellular carcinoma HepG2 cells
- Phase Behavior of Phytosterol Ethoxylates in an Imidazolium-Type Room-Temperature Ionic Liquid
- Development of a Polarized Raman Spectrometer for Supercritical Fluids Having High Critical Points
- Stable Surfactant-Free Toluene-Polyethylene-in-Water Emulsion Prepared by Ultrasonication at High Temperature
- An MO Theoretical Investigations of the Electronic Spectra of Divalent Sulfur Compounds
- Improvement of Electrical Properties of Human Phantom in Low-Frequency Band By Using Carbon Microcoil