Disruption and Overexpression of Acid Phosphatase Gene (aphA) from a Miso Koji Mold, Aspergillus oryzae KBN630, and Characterization of the Gene Product

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概要

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• An acid phosphatase gene (aphA gene) from an industrial miso koji mold strain, A. oryzae KBN630, was disrupted by using the recently developed homologous gene replacement system for this strain. The aphA gene disruption did not affect growth on steamed soybean. Acid phosphatase production decreased by approximately 20% in the aphA gene disruptants compared with that of the wild-type strain. Utilizing the promoter of the A. oryzae TEF1 gene, AphA expressed in A. oryzae was successfully secreted into the culture medium. AphA had a molecular mass of 58.0 to 65.0 kDa, a pH optimum of 4.0, and a temperature optimum of 40°C. AphA had the ability to release inorganic phosphate from GMP and IMP. This is the first report to show directly that an A. oryzae acid phosphatase has the ability to hydrolyze GMP and IMP.

• 2012-01-01

著者

• 鈴木 徹

  岐阜大院・連農・生資科

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• Suzuki Tohru

  Life Science Research Center, Gifu University

• Kusumoto K

  National Food Research Institute

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• Kuriyama Ken-ichi

  Department Of Applied Molecular Biology And Biochemistry Tokyo University Of Agriculture And Technol

• Kusumoto Ken-ichi

  National Food Research Institute

• Kitamoto Noriyuki

  Food Research Center Aichi Industrial Technology Institute

• Hasegawa Osamu

  Food Research Center Aichi Industrial Technology Institute

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• YOSHINO YASUDA

  Food Research Center, Aichi Industrial Technology Institute

• Yoshino Yasuda

  Food Research Center Aichi Industrial Technology Institute

• Suzuki Tohru

  The United Graduate School Of Agricultural Science Gifu University

• Suzuki Tohru

  Molecular Genetics Research Center Gifu University

• Kitamoto Noriyuki

  Aichi Industrial Technol. Inst. Aichi Jpn

• Suzuki Tohru

  United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.

• Kato Masashi

  Department Of Applied Biological Chemistry Fuculty Of Agriculture Meijo University

• Kato Masashi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Meijo University

• SUGIMOTO Tatsuya

  Nakamo

• Shiraishi Yohei

  Bio'c

• Kitamoto Noriyuki

  Food Res. Center Aichi Ind. Tech. Inst.

• SUZUKI Tohru

  The United Graduate School of Agricultural Science, Gifu University

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