Growth and Adhesion of Osteoblast-Like Cells Derived from Neonatal Rat Calvaria on Calcium Phosphate Ceramics

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概要

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• The effects of biocompatible ceramics on the growth and adhesion of osteoblast-rich rat calvarial cell cultures were investigated. Osteoblast-like cells and mouse fibroblast-like L-929 cells were cultured on composite sinters of hydroxyapatite (HAP) and β-tricalcium phosphate (TCP) culture carriers, whose Ca/P molar ratios were adjusted to values of 1.50, 1.55, 1.60, 1.64 and 1.67. The growth rates of both cell types were accelerated on the TCP-HAP ceramics as compared to those on polystyrene plastic (LUX) or bioinert zirconia ceramics. The population of osteoblast-like cells reached a density of 2.28×10^5 cells/cm^2 on 100% HAP (Ca/P ratio 1.67) at 9d of culture, while the corresponding cell density was 1.66×10^5 cells/cm^2 on LUX and 1.26×10^5 cells/cm^2 on zirconia. Adhesion of the osteoblast-like cells on TCP-HAP ceramics was similarly increased as compared with that on LUX or zirconia ceramics. The adhesion of L-929 cells on TCP-HAP ceramics was found to be weaker than that on cultures on LUX or zirconia ceramics. The time-dependent variations in the alkaline phosphatase activity of the osteoblast-like cells showed that the osteoblastic phenotype was potentiated by culturing the cells in calcium-rich media. The surface analyses of the Ca/p ratio and the microstructure by XRD and FTIR suggest that the Ca-rich surface was newly formed by recombination on the surface layer in the culture medium containing fetal bovine serum. These results suggest that the surface of TCP-HAP ceramics, especially that of 100% HAP ceramics, are effective for accelerating growth and differentiation of osteoblast-like cells. This is most probably due to the chemical and physical instability and composition of 100% HAP, which promote the formation of a Ca-rich layer at the cell-material interface and provision of Ca ions to the osteoblast-like cells.

• 社団法人日本生物工学会の論文
• 2000-01-25

著者

• Suzuki Takahiro

  Department Of Chemical Engineering Science Yokohama National University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• NAGATA Fukue

  National Institute of Advanced Industrial Science and Technology

• YOKOGAWA Yoshiyuki

  National Institute of Advanced Industrial Science and Technology

• OHASHI RYO

  Department of Biological Science and Technology, Science University of Tokyo

• Ohashi R

  Sci. Univ. Tokyo Chiba Jpn

• Ohashi Ryo

  Department Of Biological Science And Technology Science University Of Tokyo

• NISHIZAWA Kaori

  National Institute of Advanced Industrial Science and Technology

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• HUKKANEN MIKA

  Department of Histochemistry, Imperial College School of Medicine

• BUTTERY LEE

  Department of Histochemistry, Imperial College School of Medicine

• POLAK JULIA

  Department of Histochemistry, Imperial College School of Medicine

• Polak Julia

  Department Of Histochemistry Imperial College School Of Medicine

• Buttery Lee

  Department Of Histochemistry Imperial College School Of Medicine

• Hukkanen Mika

  Department Of Histochemistry Imperial College School Of Medicine

• Nishizawa K

  National Industrial Research Institute Of Nagoya

• Yokogawa Yoshiyuki

  National Institute Of Advanced Industrial Science And Technology (aist)

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• Suzuki Takahiro

  Department Of Anesthesiology Surugadai Nihon University Hospital

• NISHIZAWA KAORI

  National Industrial Research Institute of Nagoya

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