Distribution of Ytterbium (Yb) in Cells of Streptomyces sp. YB-1 Which Can Accumulate Yb, and Reusability of Cells and Cell Membrane as Bioadsorbent for Yb(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The cells of Streptomyces sp. YB-1 adsorbed 4-6 mg ytterbium (Yb) per g dry weight. The Yb contents of the cell wall fraction, cell-free extract, and cell membrane fraction were 11%, 2%, and 87%, respectively. The Yb content in the cell membrane fraction was 20-25 mg per g dry weight. The adsorbed Yb could be quantitatively desorbed by treating the cell membrane fraction with 1 mM EDTA and 1 M HC1 at 37℃ for 4 h. Treatment with 1 M NaOH caused Yb desorption to some extent. Treatments with proteinase K, lysozyme, 0.5% Triton X-100, 0.4% sodium dodecyl sulfate, and 1 M NaCl did not cause Yb desorption. Elemental analysis of Yb-adsorbed materials after removal of proteins and then extraction of lipids from the membrane fraction revealed that the molar ratio of Yb and P in the materials was about 1 : 1. The cells and the membrane fraction could be used repeatedly as a bioadsorbent for Yb.
- 公益社団法人日本生物工学会の論文
- 2004-09-25
著者
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鈴木 徹
岐阜大院・連農・生資科
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SUZUKI Tohru
United Graduate School of Agricultural Science, Gifu University
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Kawai K
Faculty Of Applied Biological Sciences Gifu University
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Suzuki T
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Suzuki Tohru
Life Science Research Center, Gifu University
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Suzuki Tohru
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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Kawai Keiichi
Faculty Of Applied Biological Sciences Gifu University
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Kawai K
Fac. Appl. Biol. Sci. Gifu Univ.
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Kawai Keiichi
School Of Health Sciences Faculty Of Medicine Kanazawa University
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Iwama Tomonori
Faculty of Applied Biological Sciences, Gifu University
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Pertiwiningrum Ambar
Faculty of Animal Science, Gadjah Mada University
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PERTIWININGRUM AMBAR
United Graduate School of Agricultural Science, Gifu University
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INO YOSHINAKA
Faculty of Applied Biological Science, Gifu University
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Pertiwiningrum Ambar
Faculty Of Animal Science Gadjah Mada University
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SUZUKI Tohru
Faculty of Applied Biological Sciences, Gifu University
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Iwama T
Faculty Of Applied Biological Sciences Gifu University
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Iwama Tomonori
Faculty Of Applied Biological Sciences Gifu University
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Iwama Tomonori
Fac. Appl. Biol. Sci. Gifu Univ.
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Ino Yoshinaka
Faculty Of Applied Biological Science Gifu University
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Kawai Keiichi
Fac. Appl. Biol. Sci. Gifu Univ.
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Suzuki Tohru
The United Graduate School Of Agricultural Science Gifu University
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Suzuki Tohru
Molecular Genetics Research Center Gifu University
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Suzuki Tohru
Life Science Research Center Gifu University
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Suzuki Tohru
United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.
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Kamimura Kiyohisa
Department Of Radiology Fujimoto Hayasuzu Hospital
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SUZUKI Tohru
The United Graduate School of Agricultural Science, Gifu University
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